Alexander C. Jackson, Chen Liu, Makoto Fukuda, Michael Lazarus and Laurent Gautron* 1 Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT, USA 2 Division of Hypothalamic Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA 3 Baylor College of Medicine, Childrens Nutrition Research Ct, Houston, TX, USA 4 Internat...

Potato ( Solanum tuberosum L.) cultivar IPB CP1 is suitable as a raw material for the potato chip industry. plants are sensitive to various abiotic stresses such drought, aluminium and salinity, which induce reactive oxygen species (ROS). ROS very toxic plant cells. Superoxide dismutase (SOD) one of enzymes that catalyse H2O2 O2. This study aimed establish transgenic cv. containing MmCuZn‐SOD g...

Genetic engineering is the process of modifying an organism's genetic composition by adding foreign genes to produce desired traits or evaluate function. Dr. Jon W. Gordon and Sterling Professor Emeritus at Yale Dr. Frank H. Ruddle were pioneers in mammalian gene transfer research. Their research resulted in production of the first transgenic animals, which contained foreign DNA that was passed...

Artificial modification of Bacillus thuringiensis (Bt) proteins can effectively improve their resistance to target pests, but the effect such on diversity rhizosphere microorganisms remains unclear. Transgenic maize 2A-7 contains two artificially modified Bt proteins, mCry1Ab and mCry2Ab. These enter soil pose a potential threat microbial diversity. To assess impacts bacteria communities, conte...

Two Citrus sinensis (L.) phosphomannose isomerase (PMI) genes, CsPMI1 and CsPMI2, were evaluated as novel selectable markers in mature citrus transformation. Transgenic shoots produced after transformation of Kuharske rootstock with each PMI construct selected on six treatments mannose sucrose. For CsPMI1, there no significant differences among the various sucrose for mean number positive (PS),...

Aim. The aim of the study was obtaining Camelina sativa (L.) Crantz lines with yeast genes trehalose synthesis TPS1 and TPS2 to increase their resistance drought. Methods. Seeds C. genotype FEORZhYaF-1 were used for in vitro culture establishment. For this hypocotyl segments shoot meristems 5-days-old camelina seedlings cultivated on three different nutrient media regeneration supplemented vari...

introduction: (s)- 3,5-dihydroxyphenylglycine (dhpg) is an agonist for group i metabotropic glutamate receptors. dhpg-induced synaptic depression of excitatory synapses on hippocampal pyramidal neurons is well known model for synaptic plasticity studies. the aim of the present study was to examine the effects of dhpg superfusion on excitatory synapses on pyramidal and fast-spiking gabaergic cel...

The physiological roles of the protease inhibitor SERPINB3 (SB3) are still largely unknown. The study was addressed to assess the biological effects of this serpin in vivo using a SB3 transgenic mouse model. Two colonies of mice (123 transgenic for SB3 and 148 C57BL/6J controls) have been studied. Transgenic (TG) mice showed longer survival than controls and the difference was more remarkable i...

background: phic31 integrase system provides a new platform in various felid of research, mainly in gene therapy and creation of transgenic animals. this system enables integration of exogenous dna into preferred locations in mammalian genomes, which results in robust, long-term expression of the integrated transgene.objectives: identification of a novel pseudo attp site.materials and methods: ...