Monitoring of transgenic plants in the field is important, but risk assessment has entailed laborious use of invisible marker genes. Here, we assessed three easily visible marker transgenes--green fluorescent protein (GFP), R, and Nicotiana tabacum homeobox (NTH) 15 genes--for their potential use as marker genes for monitoring genetically modified plants. Transgenic Arabidopsis thaliana plants ...

In the course of coexpressing genes for pathogenesis-related (PR) proteins for a class IV chitinase and an acidic glucanase in transgenic wheat plants, we regenerated a wheat line that developed necrotic lesions containing dead cells in the T2 and subsequent generations. Lesion spots were detected at the booting stage (5- to 6-week-old plants) in lines homozygous for the transgene loci. In cont...

Genotype specificity is a big problem lagging the development of efficient hexaploid wheat transformation system. Increasingly, the biosecurity of genetically modified organisms is garnering public attention, so the generation of marker-free transgenic plants is very important to the eventual potential commercial release of transgenic wheat. In this study, 15 commercial Chinese hexaploid wheat ...

Transgenic plants producing Bacillus thuringiensis toxins show great promise as insect control agents, provided that effective resistance management strategies are implemented early in their product cycle. To be effective, these strategies require detailed knowledge of the toxins’ mode of action and of the insect’s genetic repertoire of potential resistance genes, knowledge which is still incom...

We examined the function of the rice (Oryza sativa L.) antiporter-regulating protein OsARP by overexpressing it in tobacco (Nicotiana tabacum L.). In public databases, this protein was annotated as a putative Os02g0465900 protein of rice. The OsARP gene was introduced into tobacco under the control of the cauliflower mosaic virus 35S promoter. The transformants were selected for their ability t...

genic crops is considered a major threat to the durability of these crops (van Rie, 1991; Gould, 1994; McGaughey, Seed mixtures are recommended as a strategy to minimize or avoid 1994). Refugia in the form of susceptible (nontranshaving insects develop resistance to insect-resistant transgenic crops. genic) plants to prevent exposure of some portion of The objective of this study was to evaluat...