amorpha-4,11-diene synthase (ads) is a key enzyme in biochemical pathway of the antimalarial agent artemisinin. an agrobacterium-mediated transformation was carried out to express a synthetic ads gene in green microalga chlamydomonas reinhardtii strain 125c with bacterial strains gv3101 and lba4404. the foreign gene was optimized based on codon usage bias of the microalga. integration of the ad...

The effectiveness of commercial Bt-cotton in pest management, influence on arthropod diversity, natural enemies, and toxin flow in the insect fauna under field conditions were studied keeping in view the need to assess bioefficacy and biosafety of Bt-transgenic cotton. There were no significant differences in oviposition by Helicoverpa armigera on Bt-transgenic and non-transgenic cottons (9.2 v...

brassica napus is an important oilseed crop and the yield loss due to fungal disease stem rot caused by sclerotinia sclerotiorum is a serious problem in cultivation of this crop. the pathogenesis-related (pr) protein, glucanase, hydrolyzes a major cell wall component, glucan, of the pathogenic fungi and acts as a plant defense barrier. in this study, a β-1,3-glucanase (bgn13.1) gene was isolate...

A controlled flow of porphyrin metabolites is critical for organisms, but little is known about the control of porphyrin biosynthesis under environmental stress. We monitored transgenic rice (Oryza sativa) plants expressing Myxococcus xanthus protoporphyrinogen oxidase (PPO) for their response to drought stress. Transgenic plants showed significantly improved drought tolerance, as indicated by ...

BACKGROUND Codon usage differences are known to regulate the levels of gene expression in a species-specific manner, with the primary factors often cited to be mRNA processing and accumulation. We have challenged this conclusion by expressing the human acetylcholinesterase coding sequence in transgenic plants in its native GC-rich sequence and compared to a matched sequence with (dicotyledonous...

Delay of leaf senescence through genetic modification can potentially improve crop yield, through maintenance of photosynthetically active leaves for a longer period. Plant growth hormones such as cytokinin regulate and delay leaf senescence. Here, the structural gene (IPT) encoding the cytokinin biosynthetic enzyme isopentenyltransferase was fused to a functionally active fragment of the AtMYB...

The modified Cry1Ac was expressed in transgenic tobacco plants. To allow secretion of the Cry1Ac protein into the intercellular space, the signal peptide sequence of potato proteinase inhibitor II (pinII) was N-terminally fused to the Cry1Ac encoding region. Expression of Cry1Ac in transgenic tobacco plants was assayed with ELISA. The results showed that pinII signal peptide sequence enhanced t...

transgenic and non transgenic nicotiana tabacum l (cultivar wisconsin) containing ri t-dna were treated with 0, 0.2 and 0.4 mgl-1 ga3 in murashig and skoog medium. some physiological parameters including shoot length, leaf area, number of auxiliary bud, fresh and dry weight, number and length of trichomes were measured. shoot length, fresh weight and dry weight were increased but number of tric...