In this research, we investigated the cytotoxic mechanisms of Cochlodinium polykrikoidescell lysate on isolated rat liver hepatocytes.This micro algae is responsible for a severe and widespread harmful algal bloom in the Persian Gulf and Gulf of Oman (2008-2009). Isolated hepatocytes were obtained by collagenase perfusion of Sprague-Dawley rat liver.According to our results, incubation of algal...

Mosquito-borne diseases are illnesses caused by the transmission of viruses, parasites, and protozoa to humans mosquito vectors. Mosquitoes spread various diseases, including, dengue fever, malaria, chikungunya, West Nile Zika yellow fever. Bacterial DNA cellular proteins were shown help produce AgNPs, with bacterial acting as a stabilising agent both reducing agent. As result, lysate, which co...

Background and Objectives: In the original in vitro method for evaluation of iron bioavailability using caco-2 cell model, ferritin formation by cells is considered as a functional indicator of both absorption and utilization of iron. However, as iron is measured in the tested material and then ferritin concentration is measured in caco-2 cell lysate, it is almost impossible to have a concrete ...

Shrimp anti-lipopolysaccharide factor (SALF) is an antimicrobial peptide with reported anticancer activities, such as suppression of tumor progression. In this study, we prepared a potential cancer vaccine comprised of SALF in conjunction with the cell lysate of inactivated murine bladder carcinoma cells (MBT-2), and evaluated its efficacy in a mouse tumor model. Our study shows that SALF added...

PURPOSE The objectives of this study were to assess the toxicity and immunological response induced by the intradermal (i.d) administration of tumor lysate-pulsed dendritic cells (DCs). EXPERIMENTAL DESIGN Patients with stage IV solid malignancies were treated in cohorts that received 10(6), 10(7), and 10(8) DCs i.d. every 2 weeks for three vaccines. Each vaccine was composed of a mixture of ...

Lake sediments hold a wealth of information from past environments that is highly valuable for paleolimnological reconstructions. These studies increasingly apply modern molecular tools targeting sedimentary DNA (sedDNA). However, sediment core sampling can be logistically difficult, making immediate subsampling sedDNA challenging. Sediment cores are often refrigerated (4 °C) weeks or months be...

sertoli cell tumors of the testis are extremely rare tumors with a heterogeneous pathology. three histological variants have been described: sertoli cell tumor not otherwise specified (nos), large cell calcifying sertoli cell tumor and the sclerosing sertoli cell tumor. the sclerosing sertoli cell tumor described herein is associated with prominent stromal sclerosis. they present as painless sc...

Recent studies have reported that APC can present particulate exogenous Ag in the context of class I MHC to CD8+ CTL, and our laboratory demonstrated that IL-3 could enhance CTL generation to exogenous Ag. In this paper, we wished to determine whether presentation of particulate Ag could be enhanced by IL-3. A T cell hybridoma, B3Z86/90.14 (B3Z) restricted to Ova/Kb, was used as an indicator fo...

Approved angiogenesis inhibitors generally target certain growth factors or their receptors, which exist in both normal and cancerous cells; this results suppression of cell-signaling pathways throughout the body causes adverse effects. For reason, there is a need for new inhibitor that can specifically tumor vasculature. In our previous study, we revealed doppel, prion-like protein, was overex...