The Western blot techniques that were originally established in the late 1970s are still actively utilized today. However, this traditional method of Western blotting has several drawbacks that include low quality resolution, spurious bands, decreased sensitivity, and poor protein integrity. Recent advances have drastically improved numerous aspects of the standard Western blot protocol to prod...

To evaluate the presence and extension of West Nile virus where French soldiers are stationed in Africa, specific antibody prevalence was determined by using ELISA and Western blot. Among 245 horses living in close proximity to the soldiers, seroprevalence was particularly high in Chad (97%) and Senegal (92%).

Background Human coagulation factor IX is a 57kDa plasma serine protease made in Liver which plays a vital role in the blood coagulation cascade. FIX deficiency causes severe disorder Hemophilia B or Christmas disease. Nowadays, recombinant proteins have important roles in treatment of diseases. Although, cultivated mammalian cells because of their ability for producing properly folded protein ...

BACKGROUND The importance of specific animal allergy in immunopathology of asthma and atopic diseases remained to be defined. METHODS We measured total and specific IgE. Western blotting of some allergens was also characterised. RESULT There was a significant elevation of IgE in 3 persons among 15 samples collected. Western blotting showed common husbandry allergens from 3 sources that have...

orchard workers in north china are highly exposed to orchard pollens, especially peach and other rosaceae family pollens during pollination season. the aim of this study was to investigate whether occupational allergy to peach tree pollen as a member of rosaceae family is ige-mediated and to evaluate the cross-reactivity among rosaceae family pollens. allergen skin test and conjunctival challen...