White spot syndrome virus (WSSV) is one of the major and most serious pathogen in the shrimp industry. As one of the most abundant envelope protein, VP24 acts as a core protein interacting with other structure proteins and plays an important role in virus assembly and infection. Here, we have presented the crystal structure of VP24 from WSSV. In the structure, VP24 consists of a nine-stranded β...

Immortal cell lines have not yet been reported from Penaeus monodon, which delimits the prospects of investigating the associated viral pathogens especially white spot syndrome virus (WSSV). In this context, a method of developing primary hemocyte culture from this crustacean has been standardized by employing modified double strength Leibovitz-15 (L-15) growth medium supplemented with 2% gluco...

White spot syndrome virus (WSSV) is the causative agent of white spot syndrome, one of the most devastating diseases in shrimp aquaculture. The genome of WSSV includes a gene that encodes a putative family B DNA polymerase (ORF514), which is 16% identical in amino acid sequence to the Herpes virus 1 DNA polymerase. The aim of this work was to demonstrate the activity of the WSSV ORF514-encoded ...

The HIV transactivating response RNA-binding protein (TRBP) plays an important role in many biological processes. We have cloned three cDNAs from newly identified genes in the TRBP family from Fenneropenaeus chinensis. These genes have been designated Fc-TRBP1-3. Recombinant Fc-TRBP1, which was produced in Escherichia coli, was used for panning of a T7 phage display library of the Chinese shrim...

An experiment was conducted to explore the pathogenic effect of WSSV infection in reproduction of the Indian white shrimp, Fenneropenaeus indicus by investigating the time-course expression of female reproduction gene, namely vitellogenin (Vg) and thrombospondin (TSP), following WSSV challenge. Increased levels of expression for both Vg and TSP was observed in the infected group at 30 h post-in...

The impacts of mariculture on coastal ecosystems are increasingly raising attention recently. To study the prevalence white spot syndrome virus (WSSV) in wild crustaceans, a pathogenic agent cultured shrimps, continuous and large-scale surveys Bohai Sea were firstly conducted from 2016 to 2018. A total 820 samples collected 59 sampling sites. results loop-mediated isothermal amplification (LAMP...

white spot syndrome virus (wssv) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including iran. in the present study, a pair of primers was designed according to the sequence of vp 28 gene of wssv in the genbank. vp28 gene from an iranian wssv isolate (irvp28) was cloned, sequenced and expressed in escherichia coli bl21(de3) strain in order to produce vp28 protein...

the introduction of l. vannamei to iran was initiated when high mortality occurred in shrimp farms of abadan in  2002 then in bushehr during summer of 2004.. inspection of l. vannamei for infectious agents , specially virus and  bacteria and relationship between them were the main objectives of this study. therefore about 240 samples consisting  of shrimps of l. vannamei were collected from far...

The objective of this survey was to provide a software ecological model to predict the incidence of WSSV in Litopenaeus vannamei ponds of Gwater area located in southeastern of Iran. The coding and grouping of effective factors, which were involved in the occurrence of White Spot Syndrome Virus (WSSV) including temperature, salinity, oxygen, pH, ammonia, silica, pond preparation and management ...