S. Kakoolaki

[ 1 ] - Effects of Chlorella vulgaris on blood and immunological parameters of Caspian Sea salmon (Salmo trutta caspius) fry exposed to Viral Nervous Necrosis (VNN) virus

In the present study, the effects of Chlorella vulgaris on blood and immunological parameters of Caspian salmon (Salmo trutta caspius) before and after exposure to Viral Nervous Necrosis (VNN) virus were examined. In this regard, four treatments in triplicate were chosen. Groups included one control and 3treatments (T1, T2 and T3).  Fish in control group, T1, T2 and T3 were fed diets supplement...

[ 2 ] - The effect of created hemolymph apoptosis on WSSV Gama-vaccinated shrimp, Litopenaeus vannamei in WSSV disease control

White spot syndrome virus (WSSV) is the causative agent responsible for huge-shrimp viral epidemics in shrimp farms throughout the world. Our study was aimed to determine the effect of WSSV Gamma-vaccinated Litopenaeus vannamei on the occurrence of apoptosis. One thousand and twenty PL15 were randomly distributed among 2 treatments and two control groups. Gama-Vaccinated shrimp and non-Gamma-va...

[ 3 ] - The Status of white spot syndrome virus (WSSV) in Islamic Republic of Iran

White spot syndrome virus (WSSV), the causative virus of disease, is found in most shrimp farming areas in Iran, and it cause large economic losses to the shrimp farming industry. Shrimp aquaculture is an important industry in Iran and plays an important role in aquaculture production. The shrimp production in 2012 was more than 12000 metric tonnes and it is estimated it will reach 20000 tonnes...

[ 4 ] - Hemocytosis, a new disease in cultured shrimp, Fenneropenaeus indicus

Certain parasites can cause severe diseases in shrimp. Among these, protozoa are very important. The objective of our study was to describe the signs, prevalence, and intensity of hemocytosis, a new disease in shrimp, Fenneropenaeus indicus. The experimental design included three groups, one treatment as infected shrimp or group B and 2 controls with non-infected but exposure to White Spot Synd...

[ 5 ] - Some hematological and biochemical changes in blood serum of Grass carp (Ctenopharyngodon idella ) vaccinated with Aeromonas hydrophila following exposure to sublethal concentration of diazinon

Diazinon is commonly used for pest control in the agricultural fields in north of Iran. This study was conducted to determine the chronic toxicity of organophosphorous pesticide (Diazinon) in vaccinated fish and its effects on some hematological parameters and biochemical blood plasma profiles of Grass Carp (Ctenopharyngodon idella).This experiment was carried out in three groups. The first gro...

[ 6 ] - Chemical, microbiological and sensory evaluation of gutted kilka coated with whey protein based edible film incorporated with sodium alginate during frozen storage

The effects of whey protein (12%) and sodium alginate (0.5%) on chemical, microbial and sensory changes and shelf life in kilka during frozen storage are investigated for up to 6 months. Total bacteria count and Staphylococcus bacteria counts(2 / 51 and 1 / 44 log CFU/g) in coated samples showed decrease in comparison with control samples(3.21- 2.28 log CFU/g). Moisture of coated samples had s...

[ 7 ] - In vitro effects of Sargassum latifolium (Agardeh, 1948) against selected bacterial pathogens of shrimp

 During the last decade, shrimp aquaculture has developed rapidly in many developing countries. Antibiotic resistance is a major problem in shrimp aquaculture. Seaweeds are considered as potent source of bioactive compounds that are able to produce biological activities. The aim of this study was to evaluate the antibacterial activity of ethanolic and chloroformic crude extracts of the brown al...

[ 9 ] - Effects of Propolis on microbiologic and biochemical parameters of Rainbow trout (Oncorhynchus mykiss) after exposure to the pesticide

 Cypermethrin is a potential toxic pollutant that directly threatens the aquatic ecosystems and environment. Propolis, a natural product collected from plants by honey bees has many functions, such as antimicrobial, antioxidant, and anti-inflammatory. This study attempted to detrmine some biochemical and microbiological parameters of rainbow trout fillets after exposure to propolis and cypermet...

[ 10 ] - Experimentally pathogenesis of Aeromonas hydrophila in freshwater Crayfish (Astacus leptodactylus) in iran

 In this research the affect of A. hydrophila on pathogenesis of Crayfish A. leptodactylus was carried out. This study was designed in three groups as treatments and one group as control in triplicate with 20 Crayfish in each glass aquarium. The treatments have exposed to A. hydrophila. The concentrations of the bacteria in treatments were      , and ...

[ 11 ] - Effects of propolis on some blood parameters and enzymes in carp exposed to arsenic

 The purpose of this study was to investigate the therapeutic effects of natural products like propolis on biochemical and hematologic parameters in carp (Cyprinus carpio, Linnaeus 1758) exposed to arsenic. In this study fish were exposed to 0.01 mg/L arsenic and 10 mg/L propolis for seven days. Our results indicated that triglyceride, urea, total cholesterol, cobalt, ALT (alanine amino transfe...

[ 12 ] - The effect of different salinities on mortality and histopathological changes of SPF imported Litopenaeus vannamei, experimentally exposed to White Spot Virus and a new differential hemocyte staining method

  Shrimp farmers have been suffering from loss of production due to White Spot Disease (WSD) in the last decades. A few studies focused on the environmental factors predisposed WSD. Our study aimed to investigate the effects of the different salinities on occurrence of WSD. The treatments were 30 ppt, 40 ppt and 50 ppt in triplicate and corresponding untreated control groups. White Spot Virus (...

[ 13 ] - Immunological and histopathological changes in Penaeus semisulcatus challenged with Vibrio harveyi

 Two-hundred and sixty five green tiger shrimp juveniles (Penaeus semisulcatus) with the average weight of 7-12g were collected from Helleh farms in Bushehr province and transported to Iran Shrimp Research Center of Bushehr in October, 2009. The juveniles were acclimated for two weeks. The experiment was designed in three treatments (named 3, 4 and 5) and two controls (named 1 and 2) in triplic...

[ 14 ] - Selected morpho-chemical features of hemocytes in farmed shrimp, Fenneropenaeus indicus in Iran

The aim of the present study was to determine various types of hemocytes, total and differential hemocyte count and to show some indices of phagocytosis, including percent phagocytosis and phagocytic index in Indian white shrimp, Fenneropenaeus indicus in Iranian shrimp farms. The hemolymph was extracted from the shrimps and mixed with anticoagulant. It then stained and Yeast was added as forei...

[ 15 ] - Effects of different dietary levels of AFB1 on survival rate and growth factors of Beluga (Huso huso)

 In the present study, the impacts of various concentrations of Aflatoxin B1 (AFB1) on Beluga, Huso huso, under controlled conditions were investigated. Belugas (120±10g) were fed on diets containing 0, 25, 50, 75, and 100 ppb AFB1/kg of diet for 3 months. Results showed various levels of AFB1 do not significantly affect the specific growth ratio (SGR) (P < 0.05) of fish in different treatments...

[ 16 ] - تأثیر مکمل‌سازی پودر آویشن شیرازی (Zataria multiflora) بر شاخص رشد و فاکتورهای سرمی ماهی کپور معمولی (Cyprinus carpio)

استفاده از گیاهان دارویی می‌تواند در افزایش رشد آبزیان و ارتقاء سطح ایمنی غیراختصاصی به‌منظور افزایش مقاومت در برابر بیماری‌ها در بخش آبزی­پروری مؤثر باشد. گیاه آویشن شیرازی (Zataria multiflora) از خانواده نعناعیان یکی از این گیاهان است که بیشترین ترکیبات آن شامل تیمول و کارواکرول بوده و خواص ضد میکروبی و ضد قارچی آن به­خوبی شناخته شده است. لذا هدف از انجام این مطالعه بررسی تأثیر دوزهای مختلف خ...

[ 17 ] - Feasibility study providing a software model to predict incidence of White Spot Syndrome Virus in shrimp ponds contributed with physical and chemical factors in Iran

The objective of this survey was to provide a software ecological model to predict the incidence of WSSV in Litopenaeus vannamei ponds of Gwater area located in southeastern of Iran. The coding and grouping of effective factors, which were involved in the occurrence of White Spot Syndrome Virus (WSSV) including temperature, salinity, oxygen, pH, ammonia, silica, pond preparation and management ...

[ 18 ] - The effect of created hemolymph apoptosis on WSSV Gama-vaccinated shrimp, Litopenaeus vannamei in WSSV disease control

White spot syndrome virus (WSSV) is the causative agent responsible for huge-shrimp viral epidemics in shrimp farms throughout the world. Our study was aimed to determine the effect of WSSV Gamma-vaccinated Litopenaeus vannamei on the occurrence of apoptosis. One thousand and twenty PL15 were randomly distributed among 2 treatments and two control groups. Gama-Vaccinated shrimp and non-Gamma-va...

[ 19 ] - Hemocytosis, a new disease in cultured shrimp, Fenneropenaeus indicus

Certain parasites can cause severe diseases in shrimp. Among these, protozoa are very important. The objective of our study was to describe the signs, prevalence, and intensity of hemocytosis, a new disease in shrimp, Fenneropenaeus indicus. The experimental design included three groups, one treatment as infected shrimp or group B and 2 controls with non-infected but exposure to White Spot Synd...

[ 20 ] - The effect of different salinities on mortality and histopathological changes of SPF imported Litopenaeus vannamei, experimentally exposed to White Spot Virus and a new differential hemocyte staining method

  Shrimp farmers have been suffering from loss of production due to White Spot Disease (WSD) in the last decades. A few studies focused on the environmental factors predisposed WSD. Our study aimed to investigate the effects of the different salinities on occurrence of WSD. The treatments were 30 ppt, 40 ppt and 50 ppt in triplicate and corresponding untreated control groups. White Spot Virus (...

[ 21 ] - Selected morpho-chemical features of hemocytes in farmed shrimp, Fenneropenaeus indicus in Iran

The aim of the present study was to determine various types of hemocytes, total and differential hemocyte count and to show some indices of phagocytosis, including percent phagocytosis and phagocytic index in Indian white shrimp, Fenneropenaeus indicus in Iranian shrimp farms. The hemolymph was extracted from the shrimps and mixed with anticoagulant. It then stained and Yeast was added as forei...

[ 22 ] - The relation between temperature and salinity with WSSV occurrence in shrimp farms in Iran: An article review

Many shrimp farmers were suffering from White Spot Disease (WSD) onset in last decades. Oscillation of environmental factors could lead mortality in susceptible hosts. The results showed mortality started earliear (36h post inoculation) at 25OC in comparison to the temperature more than 29 OC as well as the salinity of 50 ppt in comparison to the lower degrees of salinities. It is concluded tha...

[ 23 ] - The possibility of VNN occurrence and the effects of nervous necrosis virus (NNV) on immunological and histopathological changes in Caspian Sea salmon (Salmo trutta caspius) fingerlings fed by Chlorella vulgaris

This study evaluated the possibility of VNN occurrence and the effects of Chlorella vulgaris on immune parameters before and after exposure to Viral Nervous Necrosis virus in Caspian Sea salmon (Salmo trutta caspius). Fish were divided into 4 treatments with three replicates. Treatments included one control group and three experimental groups (T1, T2 and T3).  Fish in control group, T1, T2 and ...

[ 24 ] - Evaluation of the antibacterial effects of Ag-Tio2 nanoparticles and optimization of its migration to sturgeon caviar (Beluga)

Effect of nano composite films to prolong the shelf life of Iranian beluga caviar was investigated at different concentrations of silver nanoparticles. In this study 38 caviar packs each containing 5 g of caviar were divided into 6 treatments with 3 replicates. The concentrations of 1000, 2000, 3000, 4000, 5000 and 6000 ppm of nanoparticles as well as a nano free pack as control were used. The ...

[ 25 ] - Socio-economic impacts of Coronavirus (COVID-19) outbreak on world shrimp aquaculture sector

The COVID-19 pandemic is now spreading throughout the world affecting the agricultural activities including fish and shrimp culture sectors. Most of the shrimp producing countries particularly in South-east Asia have been affected due to the lockdown, quarantine roles and regulations ordered by the countries, which was assigned to reduce and control the COVID-19 pandemic spreading in the globe....

[ 26 ] - Using Real-Time PCR to Detect Notifiable Viral Diseases (IPN, VHS and IHN) in Specific Pathogen Free (SPF) Rainbow Trout Broodstock Generations

The aim of this study was to identify different virulence pathogens using Real-Time PCR method for rapid and accurate detection of common viral diseases including Infectious Hematopoietic Necrosis (IHN), Viral Hemorrhagic Septicemia (VHS) and Infectious Pancreatic Necrosis disease (IPN) in rainbow trout broodstock have been the main generation of reproductive populations maintained in the natio...

[ 27 ] - Research Article: Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila

Our study was focused on characterization of dominant Bacillus bacteria isolated from the digestive tract of Oncorhynchus mykiss. After 8 weeks, the fish fed with probiotic-free diet were dissected under strictly controlled conditions. Fish intestines were eviscerated and its content diluted to 10−4 using Ringer solution. Two isolated bacteria were Gram-positive, catalase-positive, and oxidase-...

[ 28 ] - Isolation and molecular identification of Vibrio alginolyticus from cultured marine fish in farms located south provinces of Iran

Bacterial diseases are a major challenge and warning to the development of aquaculture industry. Vibriosis is caused by bacteria of the genus Vibrio, especially Vibrio alginolyticus, in warm and temperate regions, which cause severe damages to the marine fish production economy every year. The aim of the present study was to isolate and identify this bacterium from farmed marine fish with bacte...