background and objectives: the seed extract of silybum marianum contains seven flavonolignans known collectively as silymarin. these metabolites can be produced in hairy root cultures of s. marianum. the effect of different physical factors can change root biomass and silymarin production which has been investigated in the present study. methods: the effect of different physical factors of temp...

elicitation with middle-viscous chitosan (30 mg/50 ml) significantly stimulated silymarin synthesis in silybum marianum hairy root cultures. the root cultures established by infection with agrobacterium rhizogenes ar15834 showed a potential for production of silymarin. elicitation with medium molecular weight of chitosan (0, 5, 10, 20, and 30 mg/50 ml) was used in order to improve silymarin pro...

background and objectives: silymarin is a unique flavonoid complex with documented hepatoprotective properties. silybum  marianum hairy root culture as a source for producing silymarin has been an important strategy for study the cell signaling pathway. in the present investigation trichoderma strains- silybum marianum hairy root cultures interactions have been studied. methods: the effects of ...

silymarin production by hairy root culture of milk thistle (silybum marianum l. gaertn) was investigated using agrobacterium rhizogenes ar15834. hairy roots were induced by injection or inoculation of explants with a. rhizogenes. one month old hairy roots were dissected from the explants and grown in murashing and skoog (ms) liquid medium. polymerase chain reaction (pcr) using the b gene and th...

cardiac disorders remain one of the most important causes of death in the world. oxidative stress has been suggested as one of the molecular mechanisms involved in drug-induced cardiac toxicity. recently, several natural products have been utilized in different studies with the aim to protect the progression of oxidative stress-induced cardiac disorders. there is a large body of evidence that a...