We describe the characterization of Salmonella enterica serovar Typhi, isolated from the blood of patients with perforation and non-perforation typhoid fever, by a combination of conventional microbiological tests, 16S rRNA gene sequencing, and flagellin gene and CDP-tyvelose epimerase (rfbE) gene sequencing. The 16S rRNA gene sequencing showed that there were four base mutations from perforati...

Detection of Toxoplasma gondii in blood by means of the polymerase chain reaction (PCR) may facilitate early diagnosis of toxoplasmosis in different groups of patients. We evaluated this approach in 42 patients presenting with ocular or psychotic diseases by comparing the sensitivity and specificity of PCR after heat treatment using a microwave oven with a standard genomic DNA extraction method...

We compared light microscopy examination and a semi-nested multiplex PCR (SnM-PCR) assay in endemic areas of the Islamic Republic of Iran. A total of 68 individuals with malaria-positive and suspected malaria symptoms were included in the study. Giemsa-stained thick blood films were examined under a light microscope for malaria parasites in 100 and 200 fields. DNA was extracted from blood sampl...

Creating sustainable bioeconomies for the 21st century relies on optimizing the use of biological resources to improve agricultural productivity and create new products. Arbuscular mycorrhizae (phylum Glomeromycota) form symbiotic relationships with over 80% of vascular plants. In return for carbon, these fungi improve plant health and tolerance to environmental stress. This symbiosis is over 4...

The objective of the study was to evaluate the sensitivity, specificity and the positive and negative predictive value of a PCR assay for canine brucellosis diagnosis using 16S rRNA specific primers compared to serology, 2 mercaptoethanol-microtiter plate agglutination tests (2ME-MPAT) and a blood culture test. A sample of 48 dogs was divided into three groups, according to the results of blood...

There are various ways to detect the presence of compound drugs, such as heroin, cocaine, morphine in the human body. Either through a urine sample or blood sample. This study was undertaken with the aim to create a system that can detect the severity level of the effects of illegal drugs (narcotics) uses from the blood, with three different level ie minimal, moderate, and severe of the five co...

Thirty-two Hampshire-Yorkshire pigs (6 to 8 wk old) were inoculated with the Beltsville strain of Trichinella spiralis at a level of about 880 larvae/kg of body weight (about 15 kg). At about 100 kg, the pigs were slaughtered and 10-g samples of muscle and other tissues were digested in pepsin-HC1 and examined microscopically for T. spiralis larvae. The mean number of larvae recovered/gram was:...

OBJECTIVES Feline leukemia virus (FeLV) is a potentially life-threatening oncogenic retrovirus. The p27 viral core protein is produced by the virus in infected feline cells, is found in the cytoplasm in several blood cells and can be free in the serum and plasma. ELISA or particle-based immunoassay are commonly used to detect the presence of the p27 core protein in samples obtained from blood. ...