PREMISE OF THE STUDY To examine patterns of genetic diversity and test possible hybridization events, microsatellite markers were identified and characterized in Hoya ledongensis (Apocynaceae), and cross-amplification was tested in a congener, H. jianfenglingensis. METHODS AND RESULTS Based on the transcriptome data of H. ledongensis, 46 microsatellite primer pairs were randomly selected for ...

BACKGROUND It has been suggested that serological screening for coeliac disease (CD) should be performed in patients with chronic unexplained hypertransaminasaemia. AIMS To evaluate the specificity for CD diagnosis of serum IgA antitissue transglutaminase (tTG) determination in consecutive patients with chronic hypertransaminasaemia using the most widely utilised ELISA based on tTG from guine...

UNLABELLED PREMISE OF THE STUDY Polymorphic microsatellite markers were developed for the symbiotic green alga Trebouxia decolorans to study fine-scale population structure and clonal diversity. • METHODS AND RESULTS Using Illumina pyrosequencing, 20 microsatellite primer sets were developed for T. decolorans. The primer sets were tested on 43 individuals sampled from four subpopulations i...

Background and aims: Coeliac disease (CD) is a multifactorial disorder which has an autoimmune component characterised by the occurrence of disease specific autoreactive antibodies against the enzyme tissue transglutaminase (tTG). The aim of this study was to investigate whether binding of antibodies to the enzyme influences tTG activity. Methods: tTG activity was assayed in the presence of imm...

Genus and Species. Sus scrofa. Locus. Porcine connexin 37 (CX37) gene. Source and Description of Primers. A set of primers (F1, R1) was designed from the porcine CX37 mRNA sequence (GenBank Accession no. X86024) as well as an additional reverse primer (R2) from human (GenBank Accession no. 6093424) and murine (GenBank Accesion no. NM_008120) CX37 consensus sequence. The F1 and R2 primers were u...

Eight published microsatellite primer sets were tested on six populations of Triatoma dimidiata from Guatemala. Three of the eight resulted in amplification useful for population genetic studies. In a preliminary analysis with these three primer sets, there appears to be genetic structuring in some populations of Triatoma dimidiata and evidence of migration among populations. The use of microsa...

BACKGROUND Celiac disease (CD) is an intestinal inflammatory condition that develops in genetically susceptible individuals after exposure to dietary wheat gliadin. The role of post-translational modifications of gliadin catalyzed by tissue transglutaminase (tTG) seems to play a crucial role in CD. However, it remains to be established how and where tTG is activated in vivo. We have investigate...

UNLABELLED • PREMISE OF THE STUDY The development of microsatellite primers in the endangered species Dracaena cambodiana will be the foundation for genetic and conservation studies of D. cambodiana and several Dracaena species. • METHODS AND RESULTS A total of 26 microsatellite markers were developed in Chinese populations of D. cambodiana, using the Fast Isolation by AFLP of Sequences Con...

this study was carried out in order to evaluate allelic diversity of microsatellite markers in qtl region for salinity tolerance on chromosome 10 in rice and evaluation of agronomical traits of some iranian rice cultivars in salinity stress along with two tolerant (pokkali) and sensitive (ir29) check varieties, and association analysis for morphological traits using microsatellite markers.based...