Density functional theory in Firefly is implemented for restricted, unrestricted, and restricted-open wavefunctions. For each wavefunction, energies and analytical gradients are available. Analytical Hessians are currently not available. Instead, Hessians can be calculated in a semi-numerical fashion. Double hybrid functionals are an exception – for these functionals, only energies are currentl...

As a key factor of logistics distribution, vehicle routing problem (VRP) is a hot research topic in management and computation science. In this paper the principle and algorithm process of firefly algorithm are introduced in detail. Then the algorithm process and solving steps are designed for vehicle routing problem with time windows (VRPTW), including the coding and design of disturbance mech...

Scheduling jobs on computational grids is identified as NP-complete problem due to the heterogeneity of resources; the resources belong to different administrative domains and apply different management policies. This paper presents a novel metaheuristics method based on Firefly Algorithm (FA) for scheduling jobs on grid computing. The proposed method is to dynamically create an optimal schedul...

We developed an ultrasensitive bioluminescence assay of ATP by employing (i) adenylate kinase (ADK) for converting AMP + ATP to two molecules of ADP, (ii) polyphosphate (polyP) kinase (PPK) for converting ADP back to ATP (ATP amplification), and (iii) a commercially available firefly luciferase. A highly purified PPK-ADK fusion protein efficiently amplified ATP, resulting in high levels of biol...