conclusions according to our results, blatem-116 was the most frequent isolated esbl gene among the p. aeruginosa strains isolated from patients. results ciprofloxacin and piperacillin were the most efficient antipseudomonal agents. the results disclosed that 19 (16.37%) of the isolates were multidrug resistant and 8 (6.89%) were esbl-positive. of the 116 isolates, 30 (25.86%) were resistant to...

The emergence of plasmid mediated extended spectrum b-lactamases (ESBLs) in members of family Enterobacteriaceae has become a worldwide problem. Many clinical microbiology laboratories make no effort to detect ESBL production by Gram negative bacilli and have problem to detect ESBL mediated resistance. The choice of optimal laboratory method for detection and surveillance of ESBL producing orga...

Escherichia coli (E. coli) is a common opportunistic pathogen for nosocomial infection. The aim of the study was to examine the phenotype, genotype and epidemiology of plasmid-mediated AmpC β-lactamases in E. coli. In total, 96 clinical isolates of repeated E. coli were collected from different hospitals between August and October 2012. Using a cefoxitin disk diffusion method to identify the ph...

There is a global increase in infections caused by Enterobacteriaceae with plasmid-borne β-lactamases that confer resistance to third-generation cephalosporins. The epidemiology of these bacteria is not well understood, and was, therefore, investigated in a selection of 636 clinical Enterobacteriaceae with a minimal inhibitory concentration >1 mg/L for ceftazidime/ceftriaxone from a national su...

Extended-spectrum -lactamases (ESBLs), such as the plasmid-mediated class A TEMand SHV-type enzymes, have developed by stepwise mutations in their structural genes, resulting in either single or multiple amino acid changes in the encoded enzymes, and these changes sufficiently remodel the active site to allow attack on aminothiazolyl compounds. Since they were first identified at the beginning ...

AmpC β-lactamases are cephalosporinases that hydrolyze cephamycins as well as other extended-spectrum cephalosporins and are poorly inhibited by clavulanic acid. Although reported with increasing frequency, the true rate of occurrence of AmpC β-lactamases in different organisms, including members of Enterobacteriaceae, remains unknown. The present study was designed to determine the occurrence ...

The early eighties saw the introduction of a new group of antibiotics known as the carbapenems, which exhibited broader spectrum of activity as compared to cephalosporins. These group of antibiotics were active against Gram negative bacilli including non-fermenters like Pseudomonas producing extended spectrum betalactamases (ESBLs) and AmpC betalactamases (Deshpande et al., 2004; Gladstone et a...

AmpC beta-lactamases are cephalosporinases that confer resistance to a wide variety of beta-lactam drugs and that may thereby create serious therapeutic problems. Although reported with increasing frequency, the true rate of occurrence of AmpC beta-lactamases in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis remains unknown. We tested a total of 1,286 consecutive, nonrepeat isol...