We investigated the influence of different conditions (light composition and plant growth regulators (PGRs) in culture media) on morphophysiological parameters Stevia rebaudiana Bertoni vitro vivo. Both PGRs light spectra applied were found to significantly affect morphogenesis. During micropropagation stage S. rebaudiana, optimal growth, with a multiplication coefficient 15, was obtained an MS...

Nidularium minutum is an ornamental bromeliad from the Brazilian Rainforest. The micropropagation of this species essential for obtaining plants available conservation programs or commercial use. Our study aimed to establish efficient plant production method by in vitro sprouting. This takes a long time sprout vitro, and 10% produce shoots culture medium without growth regulators (PGRs). When s...

An efficient plant regeneration method was established for Caesalpinia bonduc (L.) Roxb. by culturingimmature epicotyl explants. Morphogenic calli were initiated from 96% of epicotyl explants on MS mediumsupplemented with BA (6-benzylaminopurine) 4.0 mg/l and NAA (Naphthalene acetic acid) 1.0 mg/l. The calliformed were excised and subcultured on MS medium fortified with 1 mg/l...

Here, we compared miRNA expression profiles in embryonic cell cultures of the conifer Picea balfouriana following application of the synthetic cytokinin 6-benzylaminopurine (6-BAP). We used next-generation sequencing to analyze three libraries of small RNAs from the treated embryogenic cell cultures and generated 24,000,000 raw reads from each of the libraries. Over 70 differentially regulated ...

The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared proteomes of tissue cultures from leaf explants of these two lines. Both 2HA and Jemalong explants were grown on media containing the auxin 1-naphthaleneacetic acid and the cytokinin 6-benzylaminopurine. Proteins were extracted from ...