A new strategy for construction of synthetic gene encoding human basic fibroblast growth factor comprising DNA annealing-ligation and augmentation by polymerase chain reaction was introduced. The sequence of the gene and corresponding amino acid chain were modified in order to increase stability of the protein. First, 300 bp and 160 bp fragments of the gene were assembled from 18 oligonucleotid...

calpastatin (cast) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. in the present study the polymorphism of the cast gene ofkhalkhali goat in azerbaijan province in iran was investigated by polymerase chain reaction and restriction fragment length polymorphism technique (pcr-rflp). genomic dna was extracted from whole blood samples collected from 200 kh...

AIM This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella antigens in Egypt. MATERIALS AND METHODS The phenotypic characterization of Salmonella strains was done using standard microbiological, biochemical,...

in order to investigate the presence of barley mild mosaic virus, 425 samples of barley and wheat from razavi khorasan, northern khorasan, golestan, yazd and western azarbayjan were collected in spring 2011. plolyclonal antiserum was used in order to detect baymv by das-elisa. the highest level of baymv infection was found in azarbayjan gharbi and detect inrazavi khorasan, northern khorasan and...

The fidelity of in vitro DNA synthesis catalyzed by the large fragment of DNA polymerase I was examined. The templates, specifically designed to detect shifts to the +1 or to the -1 reading frame, are composites of M13mp8 and bacteriophage T4 rIIB DNA and were designed to assist in the identification of the types of frameshifts that are the specific consequence of DNA polymerization errors. In ...

A factor IX polymorphism 5' to exon 2 was described as 1.70 versus 1.75 kb bands on Southern blots of Ddel digests (1). By primer directed polymerase chain reaction (PCR) amplification, the two alleles are readily apparent on agarose electrophoresis of undigested fragments (430 or 380 bp); in heterozygous women or amplified mixtures of two hemizygous male types, an additional fragment (~480 bp)...

BACKGROUND Salmonella spp. is the major bacterial pathogen in poultry and is responsible for significant economic losses of the poultry industry in many parts of the world. Among Salmonella spp., Salmonella gallinarum and Salmonella. pullorum are the most common causative agents of chicken salmonellosis resulting in high mortality and morbidity. OBJECTIVES The aim of this study was to identif...

Mercury resistant (Hgr) bacteria were isolated from four terrestrial sites: three containing high levels of mercury (sites T2, SE, and SO) and one uncontaminated site (SB). The frequencies of Hgr bacteria in the total cultivable populations were 0.05% (SB), 0.69% (SO), 4.8% (SE), and 25% (T2). Between 35 and 100% of the isolates from the four sites contained DNA sequences homologous to a DNA pr...