Background: Chromomycin A3 (CMA3) staining, with the slide method or fluorescence microscopy, is widely used for indirect assessment of protamine deficiency in a semen sample. Flow cytometry is the most suitable tool which to improve assessment accuracy, both in terms of statistical analysis and to prevent observer variation. This study provides a simple procedure to account for merocyanine 540...

background: it has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. these methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques. objective: this study compared simple density gradient methods and a combin...

perrault's syndrome (p.s.) is rare. the combination of gonadal dysgenesis and hearing loss was accompanied by 46,xxkaryotype in three sisters with parental consanguineous marriage. genetic investigation revealed normal female karyotype, positive liarr and negative fluorescence ipj-bodies, which was confirmed by molecular study on the basis of fluorescence in situ hybridization (pish), with appl...

abstract- greenhouse experiment was conducted in factorial experiment arranged as a completely randomized design (crd) to evaluate the effect of glomus intraradices, pseudomonas fluorescence and soil drying on different forms of potassium (k) and the changes of clay minerals in a calcareous soil after maize planting. treatments consisted of arbuscular mycorrhizal (am) fungus at two levels: g0 (...

the changes of chl a fluorescence induction curve in response to different salinity levels in canola (barassica napus, l.) plants were investigated. the canola seedlings were exposed to salt stress (100 mm and 200 mm nacl) for 7 days and chl a fluorescence induction kinetics was analysed. by using the ojip-test, various parameters like vj, area, n, φpo, φeo, ψo, rc/abs and piabs were calculated...

Several heterocyclic bioactive fluorescent 3-alkyl-3H-pyrazolo[4,3-a]acridin-11-carbonitriles were conveniently synthesized from the reaction of 1-alkyl-1H-indazoles with different aryl acetonitrile in basic methanol solution in good yields. The interactions of 3H-pyrazolo[4,...