PURPOSE To assess the effects of LASIK with the 15-kHz IntraLase femtosecond laser on corneal endothelium. METHODS In a prospective, single-center clinical trial, 138 patients (mean age: 32.0 ± 7.1 years [range: 21 to 42 years]) underwent femtosecond LASIK for the correction of myopia -0.75 to -9.00 diopters (D) and cylinder up to 3.25 D. Patients were divided into two groups: contact lens gr...

Basement membranes influence growth, shape and differentiation of cells and tissues. However, the role and influence of Descemet's membrane during corneal development is not understood. To address this question, the relationships between cell growth and fibronectin, laminin and actin distribution in the developing rat corneal endothelium in vivo has been examined. During fetal development, rat ...

Particulate fractions from fresh bovine corneal endothelium exhibited high affinity, specific binding by a potent muscarinic cholinergic radioligand, [3H]QNB. Particulate fraction binding sites exhibited half maximal binding at approximately 0.3 nM [3H]QNB and reached a maximal binding capacity of 820 fmoles/mg of protein at 3 nM [3H]QNB. Muscarinic cholinergic antagonists and agonists competed...

Microscopic lesions of corneal endothelium produced by herpes simplex virus were studied in flat preparation of the endothelium following injection of the virus into the anterior chamber of the rabbit eye. The intraocular injection of the virus produced corneal opacity, characteristic endothelial lesions, and uveitis. These ocular changes were virus specific, and were accompanied by virus multi...

Scanning electron microscopy of the endothelium of experimental disciform keratitis revealed corneal endothelial changes which distinguished disciform oedema from the more progressive stages of disciform keratitis. The endothelium of corneas with disciform oedema were wholly intact and characterised by subtle morphological alterations. In contrast, the more progressive stages of disciform kerat...

PURPOSE In this study, the involvement of signaling pathways in the regulation of HCO(3)(-) permeability across the apical membrane of the corneal endothelium was examined. METHODS Cultured bovine corneal endothelial cells (CBCECs) were grown to confluence on permeable membranes. Apical and basolateral sides were perfused with a HCO(3)(-)-rich Cl(-)-free Ringer's solution (28.5 mM; pH 7.5). R...

PURPOSE Junctional adhesion molecules (JAMs) are a family of adhesion proteins found in intercellular junctions. Evidence suggests that JAM-A is important for the regulation of tight junction assembly and epithelial barrier function. The authors recently reported that JAM-A is expressed in rabbit corneal endothelium and that antibody to JAM-A produces corneal swelling. In the present study, the...

The objective of this study was to collect a comprehensive database of ocular tissue permeability measurements found in a review of the literature to guide models for drug transport in the eye. Well over 300 permeability measurements of cornea, sclera, and conjunctiva, as well as corneal epithelium, stroma, and endothelium, were obtained for almost 150 different compounds from more than 40 diff...