BACKGROUND Cryptosporidium epidemiology is poorly understood, but infection is suspected of contributing to childhood malnutrition and diarrhea-related mortality worldwide. METHODS/FINDINGS A prospective cohort of 108 women and their infants in rural/semi-rural Tanzania were followed from delivery through six months. Cryptosporidium infection was determined in feces using modified Ziehl-Neels...

Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and dog). Significant diversity was observed among species and genotypes in the primer and target regi...

BACKGROUND: Oocysts of Cryptosporidium species are frequently found in surface water. Approved detection methods, such as EPA method 1623, do not discriminate between infectious and dead oocysts. Culture of C. parvum and C. hominis, the two Cryptosporidium species responsible for most human infections, has been shown to be a good alternative to animal models for detecting infective oocysts. The...

the protozoan intestinal parasite cryptosporidium commonly infects cattle throughout the world and iran. the present study was undertaken to determine the abundance and associated risk factors of cryptosporidium infection in cattle herds of northwestern iran. a total number of 246 fecal samples from 138 (56.1%) diarrheic (d) and 108 (43.9%) non-diarrheic (nd) cattle were randomly collected and ...

Established methods for quantifying experimental Cryptosporidium infection are highly variable and subjective. We describe a new technique using quantitative real-time PCR (qPCR) that can be used to measure in vitro and in vivo laboratory infections with Cryptosporidium. We show for the first time that qPCR permits absolute quantification of the parasite while simultaneously controlling for the...

The protozoan intestinal parasite Cryptosporidium commonly infects cattle throughout the world and Iran. The present study was undertaken to determine the abundance and associated risk factors of Cryptosporidium infection in cattle herds of northwestern Iran. A total number of 246 fecal samples from 138 (56.1%) diarrheic (D) and 108 (43.9%) non-diarrheic (ND) cattle were rando...

  Cryptosporidium species are one of the most common causes of gastrointestinal infection in humans around the world. This study has aimed to investigate the hyper variable region of the 18S rRNA gene in Cryptosporidium for exact parasite identification .   DNA was extracted from 26 fecal samples from which initially Cryptosporidium oocysts were identified by Ziehl-Neelsen acid-fast , Auramine ...

Ultrasound in a liquid phase cause mass and heat transfer across the liquid through cavitational processes which act as nanoreactors to generate unstable mechanical equilibrium. The effect of 1 MHz ultrasound on the inactivation of Cryptosporidium parvum was investigated. Continuous irradiation of ultrasound (20 min) increased temperature due to cavitational phenomena. Ultrasound irradiation of...

aim : the aim of the present study was to determine the species and genotypes of cryptosporidium spp. among children with diarrhea by pcr- rflp using the trap-c2 gene. background : cryptosporidium is a globally distributed protozoan parasite and one of the most common causes of infection and diarrhea in humans. patients and methods : four hundred and sixty nine stool samples were collected from...