Ethylenediaminetetraacetic acid (EDTA), when used as a main chelator for complex plant microfertilizers, causes many negative environmental effects; therefore, new compounds or use of the known to replace EDTA have been widely studied. In present study, biodegradation rate, (Raphanus sativus) growth stimulation and ecotoxicity towards Daphnia magna Chlorella vulgaris iminodisuccinic (IDS), cons...

The extraction of nucleic acids with phenol and chloroform is the standard method to remove proteins and lipids from cellular digests. A major drawback to the use of these organic solvents is their caustic and toxic qualities. Phenol can cause severe burns while chloroform is hepatotoxic and carcinogenic. We describe in this paper the use of a special blood collection tube to isolate the offens...

Phosphatidylcholine vesicles harboring the lipophilic metal ion carrier A23187 in the bilayer and encapsulating ethylenediamine tetraacetic acid (EDTA) exhibit broadly varied, pH-dependent affinity for Cu2+, Pb2+, Cd2+, and Ca2+ at micromolar levels. Initial rates of uptake by these metal-sorbing vesicles (MSVs) is strongly dependent on the metal ion affinity of A23187 and the dissociation rate...

The synthetic chelating agent EDTA can mobilize radionuclides and heavy metals in the environment. Biodegradation of EDTA should reduce this mobilization. Although several bacteria have been reported to mineralize EDTA, little is known about the biochemistry of EDTA degradation. Understanding the biochemistry will facilitate the removal of EDTA from the environment. EDTA-degrading activities we...

OBJECTIVE The aim of this study was to compare and evaluate three methods of DNA extraction for the amplification of Chlamydia trachomatis in uterine cervical samples collected in PreservCyt solution. ThinPrep is the trade name for the slide preparation. METHODS Thirty-eight samples collected in LCx buffer medium, which were identified as C. trachomatis infected by ligase chain reaction (LCR)...

The study was conducted to investigate the effects of ethylenediaminetetraacetic acid (EDTA) on seedling emergence, morphological characteristics, and photosynthetic pigments of Chenopodium album (L.) in lead (Pb) contaminated soil. The Pb contaminated soils were taken from Hirmand River. The soil samples mixed with 0.71 g Li-1 Pb (PbNO3)2 to increase the Pb concentration. EDTA was added to the...

This study measured excretion of 11 minerals induced by EDTA chelation therapy in 25 adults (aluminum, cadmium, calcium, chromium, copper, iron, lead, magnesium, manganese, mercury, zinc). We administered 20 EDTA infusions (3 g, with magnesium and ascorbate) 1 to 2 weeks apart and measured 24-hr urinary excretions of minerals before treatment and after treatments 1, 10, and 20. A modest supplem...

A chemical absorption-biological reduction integrated process can be used to remove nitrogen oxides (NOx) from flue gas. In such a process, nitric oxide (NO) can be effectively absorbed by the ferrous chelate of ethylenediaminetetraacetate (Fe(II)EDTA) to form Fe(II)EDTA-NO, which can be biologically regenerated by denitrifying bacteria. However, in the course of these processes, part of the Fe...