The activity of phospholipase C in crude enzymatic preparation of Melia azadiracht seeds (Neem seeds) was studied by the use of lecithin as a substrate in aqueous medium. The enzyme activity was found optimum at pH 2.5 and temperature 35?C. The phospholipase C was found heat labile, being inactivated 88% within 10 minutes at 90°C

Enzymes are the vital activators in life processes, likewise in the soil they are known to play a substantial role in maintaining soil health and its environment. The enzymatic activity in the soil is mainly of microbial origin, being derived from intracellular, cell-associated or free enzymes. A unique balance of chemical, physical, and biological (including microbial especially enzyme activit...

The excessive use of chemical fertilizers can lead to severe environmental damages. In recent decades, the application biostimulants improve soil composition and stimulate plant growth has contributed significantly preservation. this paper, we studied effect a rhizogenic biostimulant, obtained from fulvic acids, probiotics, prebiotics, on fertility two types soils, sandy loam in which turfgrass...

18 The objective of this study was to relate the earthworm assemblage structure with three 19 different soil use intensities, and to indentify the physical, chemical, and 20 microbiological soil variables that are associated to the observed differences in the 21 earthworm assemblage structure between soils. Three soil uses were evaluated: 1Fifty 22 year old naturalized grasslands, low use inten...

plants and soil microbes are the main sources of most enzymes available in the environment. it is, therefore, anticipated that any change in the growth of soil biota in response to salinity, may reduce the synthesis and production of extra- and intra- cellular enzymes. as a result, a reduction in the growth of plants and the activity of soil microbes in saline environments may lessen the level ...

L-Asparaginase has remarkable properties which make it useful in dual pharmaceutical and food industries.In this study, simple and advantageous method has been validated for rapid and precise determination of intracellular L-Asparaginasein bacterial species. A suspension of bacterial cells was used instead ofcell extract and incubated by substrate (asparagine) after simple wash and centrifugati...

Synchronous effects of biochar on heavy metals stress, microbial activity and nodulation process in the soil are rarely addressed. This work studied effects, under greenhouse conditions, selected Cd2+, Pb2+ Ni2+ soybean plants grown two different soils amended with biochar, their effect enzymatic activity. As a result interference between overcame metal problems maintained population major grou...