Abstract Eukaryotic cells are complex systems compartmentalized in membrane-bound organelles. Visualization of organellar electrical activity living requires both a suitable reporter and non-invasive imaging at high spatiotemporal resolution. Here we present hVoS org , an optical method to monitor changes the membrane potential subcellular membranes. This takes advantage FRET pair consisting vo...

We have developed a spectral inversion method for three-dimensional tomography of far-red and near-infrared fluorescent proteins in animals. The method was developed in particular to address the steep light absorption transition of hemoglobin from the visible to the far-red occurring around 600 nm. Using an orthotopic mouse model of brain tumors expressing the red-shifted fluorescent protein mC...

A new fluorescent acridine orange derivative, 3-amino-6-methoxy-9-(2-hydroxyethylamino) acridine (AMHA), has been applied to Hela cells in order to set up appropriate conditions for the detection of mycoplasma contaminations. Since AMHA staining reveals intensely fluorescent nuclei and slight fluorescent cytoplasm, we can visualize and localize mycoplasma contamination on each cell. In combinat...

Six fluorescent compounds were isolated and characterized in rhizomorph of A. mellea and were presented first as: F , F , F , MF , MF and MF . These compounds differed by Rf on chromatoplaque, max in UV spectra and ex, em and max in fluorescence spectra. Their GC-MS analysis indicated that F has a phenolic moiety and the other five are the benzenic derivatives. F compound was more f...

Fluorescence resonance energy transfer (FRET) efficiency measurements based on acceptor photobleaching of yellow fluorescent protein (YFP) are affected by the fact that bleaching of YFP produces a fluorescent species that is detectable in cyan fluorescent protein (CFP) image channels. The presented quantitative measurement of this conversion makes it possible to correct the obtained FRET signal...