Many recent studies have shown that glycosylation patterns of Agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. To achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. To isolate this promoter, PCR with specific primers was perform...

to characterize the distribution of melanin producing medicago-nodulating rhizobia in western and north-western of iran, 982 sinorhizobium isolates were trapped from a combination of three alfalfa populations (hamedani, nikshahri, kodi) and soil samples from eight sites across kurdestan, kermanshah, eastern azarbayjan and lorestan provinces. all rhizobial isolates were examined for their abilit...

Staphylococcus aureus carrying PVL gene remain major health problem associated with highly virulent infections. Characterization of such gene is important to know the impact and the functional significance of nucleotide variations. PCR and standard sequencing were performed for twelve Sudanese strains from different sources. Protein structures prediction, modeling and physiochemical analysis we...

avian infectious bronchitis is an acute and highly contagious disease that mainly causes respiratory symptoms in poultry. a number of serotypes and variants of the viral agent with poor cross-protection are the major problem to achieve desired immunity from vaccination. the s1 subunit of s glycoprotein (spike) is the major determinant of ibv so that a minor change in amino acid sequence of this...

sequence data of a recent coi barcoding study on fish from the nayband national park in the persian gulf showed relatively high sequence divergence among nominal gerres filamentosus specimens. the sequences grouped into two clusters, signaling the possible existence of cryptic species. the clustering pattern was corroborated by results of 16s rdna sequencing. we investigated the hypothesis that...

pasteurella multocida is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. a polymerase chain reaction (pcr) assay was developed using primers derived from conserved part of 16s-23s rrna gene. the pcr amplified a fragment size of 0.7 kb using dna from nine avian p. multocida  isolates...

A partial sequence of the mtDNA ND5 gene region was used for population study in Persian sturgeon (west and east areas of southern Caspian Sea). The result showed that although this approach was informative for phylogenetic study in sturgeon, it was less informative for population study in Persian sturgeon. 

Objectives: Royal jelly (RJ), a secretion of both the hypopharyngeal and mandibular glands of nurse workers, is believed to play a central role in honeybee queen development. Important component of royal jelly are proteins which form about 50% of the dry mass of RJ. Major royal jelly proteins (MRJPs) are the dominant proteinaceous component of royal jelly and constitute about 82-90% of total pr...

Background: Expressed sequence tags (ESTs) are sequences of cDNA fragments prepared from different tissue sources. There are over one million of these sequences in the publicly available database, and these sequences are believed to represent more than half of all human genes. The ESTs belong to different cDNA libraries, was prepared from one particular cell type, organ, or tumor. Therefore, th...

P48 protein of Mycoplasma agalactiae is used to diagnose infection and was identified as potential vaccine candidate. According to the genetic nature of mycoplasma and variable sensitivity in P48-based serological diagnosis tests, intra species variation of P48 nucleotide sequence investigated in 13 field isolates of difference province of Iran along with three vaccine strains. Samples were col...