leishmaniasis include several clinical manifestations, mostly cutaneous, visceral and mucosal (1, 2). various species can lead to diverse clinical presentations. thus species identification contribute to proper management (3). localized leishmania lymphadenitis (lll) is a distinct entity in clinicopathologic field presenting with isolated lymphadenitis and possible cocomitant cutaneous lesion i...

in this study, the full-length m2 gene of the avian influenza virus (h9n2) was isolated, analyzed and studied in detail. total rna was extracted and cdna of the m2 mrna was obtained by reverse transcriptase polymerase chain reaction (rt-pcr) using random hexamer oligoes; specific primers were used for amplification of the m2 open reading frame (orf) region. pcr was able to amplify the desirable...

  Genetic typing methods of T. gondii strains have been extensively perfected in recent years. From a technical point of view, many tools usable for genetic studied on single-copy loci have been used: RFLP, PCR-RFLP, sequencing, RAPD-PCR and isoenzyme analysis. We described the cloning and sequence analysis of the gene which encodes the major surface antigen (SAG1 or P30) of T. gondii. SAG1 is ...

Aspergillus fumigatus is a saprophyte fungus, widely spread in a variety of ecologicalniches and the most prevalent aspergilli responsible for human and animal invasiveaspergillosis. The first step to develop novel and efficient therapies is the identificationand understanding of the key tolerance and virulence factors of pathogens. The mainfocus of the present study is to perform the similarit...

introduction: follicle stimulating hormone (fsh) is one of the pituitary glycoproteines that it consists of two subunits alpha and beta. the beta subunit is responsible for the biological activity of fsh. the aim of present study was isolation of the β subunit coding sequence containing its signal sequence from human genome and then cloning of the isolated sequence in ppic9 shuttle vector under...

medicinal use of the opium poppy (papaver somniferum l) has ancient history, but the isolation of morphine was not described until the early nineteenth century. morphine is the most important alkaloid of opium poppy in the last 50 years. in the morphine pathway has been reported to generate morphine in this species, codm has a crucial role as the gene coding the enzyme responsible for demethyla...

genetic typing methods of t. gondii strains have been extensively perfected in recent years. from a technical point of view, many tools usable for genetic studied on single-copy loci have been used: rflp, pcr-rflp, sequencing, rapd-pcr and isoenzyme analysis. we described the cloning and sequence analysis of the gene which encodes the major surface antigen (sag1 or p30) of t. gondii. sag1 is th...