Background: Higher prevalence of ESBL producers is alarming in dairy sector. So limiting antimicrobial use may curtail the selection and persistence predominant conjugative plasmids among strains. The study was aimed to determine occurrence extended-spectrum beta-lactamase (ESBL) producing E. coli as well their genetic diversity, resistance integrons bovines. Methods: A total 180 faecal samples...

Background and Objective: A large group of genes associated with extended-spectrum beta-lactamases (ESBL) and AmpC expression is involved in inducing antibiotic resistance in various bacteria. Identifying these genes and assessing their harboring will be crucial in determining the pattern of antibiotic resistance. This study aimed to characterize genetic variants in extended-spectrum beta-lacta...

Members of the genus Aeromonas that commonly occur in various aquatic ecosystems are taken into account as vectors spreading antibiotic resistance genes (ARGs) in the environment. In our study strains of Aeromonas spp. (n = 104) not susceptible to ampicillin were isolated from municipal sewage of different levels of purification - raw sewage, activated sludge and treated wastewater. The crucial...

defined by susceptibility to all of ciprofloxacin, co-trimoxazole and gentamicin) isolates was low (2.9%, 5/175). PCR and sequencing showed that the only extended-spectrum b-lactamase (ESBL)-producing O25b-ST131 isolate had blaCTX-M-14. Our results showed that O25b-ST131 exhibited a wide range of susceptibility patterns. Similar to previous studies, our findings showed that O25b-ST131 isolates ...

The aim of this study was to determine the prevalence of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolated from urinary tract and bloodstream infections in a rural hospital in Manhiça, Mozambique. ESBLs were investigated among ceftriaxone-non-susceptible K. pneumoniae clinical isolates recovered between 2004 and 2009. Characterisation of blaCTX-M, blaSHV, blaOXA and ...

INTRODUCTION Pseudomonas aeruginosa is as an important opportunistic human pathogen, which is associated with several clinical infections that are usually difficult to treat because of resistance to multiple antimicrobials. The production of extendedspectrum ß-lactamases (ESBLs) is an important mechanism of ß-lactam resistance. The aims of this study were to determine the prevalence of ESBLs, a...

OBJECTIVES The objectives of this study were to determine the prevalence of carriage of ESBL-producing Enterobacteriaceae (ESBL-E) in a representative sample of the general adult Dutch community, to identify risk factors and to gain understanding of the epidemiology of these resistant strains. METHODS Adults enrolled in five general practices in Amsterdam were approached by postal mail and as...

resistance to oxyimino cephalosporins antibiotics in enterobacteriaceae is primarily done by the extended spectrum β-lactamases (esbls). clear identification of risk factors for esbl-producing infections is necessary. therefore, efficient strategies can be developed to decrease outbreak of these infections. the aim of this study was to determine the antibacterial susceptibility and esbls patter...

Methods: E. coli was isolated from 388 broiler chicken caecal samples from 22 abattoirs and from boot swabs from 442 turkey flocks over successive 1 year periods. CHROMagar ECC with and without cephalosporin antibiotics was used as isolation medium and the chicken study also used CHROMagar CTX. ESBL phenotype isolates were tested for the presence of blaCTX-M, blaOXA, blaSHV, blaTEM and ampC gen...

We constructed a phylogenetic analysis of class A beta-lactamases and found that the blaCTX-Ms have been mobilized to plasmids approximately 10 times more frequently than other class A beta-lactamases. We also found that the blaCTX-Ms are descended from a common ancestor that was incorporated in ancient times into the chromosome of the ancestor of Kluyvera species through horizontal transfer. C...