The exact metabolic relationship between insulin and glucagon is still a matter of controversy. Some authors (2,3) have proposed that glucagon, like insulin, enhances the peripheral utilization of glucose. However, much evidence has been obtained which suggests that glucagon antagonizes the action of insulin. The results obtained by Thorogood and Zimmerman (4) suggested that, since alloxan-diab...

The biosynthesis of glucagon was studied by using the recirculated, isolated perfused rat pancreas. [3H]Tryptophan was initially incorporated into acid-ethanol-extractable protein, which on gel filtration was eluted with a molecular weight ofabout 9000 and contained a small amount of glucagon immunoreactivity. With longer incubation [3H]tryptophan incorporation into a second peak was obtained i...

The kinetic characteristics of glucagon action and binding as functions of hormone and nucleotide concentrations have been investigated in the hepatic adenylate cyclase system. Either App(NH)p (S’-adenylylimidodiphosphate), 0.1 mu, or ATP, 2 mu, was used as substrate. The formation of adenosine 3’:5’-monophosphate (cyclic AMP) in response to glucagon exhibits lag times as long as 4 min in the p...

The plasma glucose response to an intravenous bolus of glucagon was examined in patients with obstructive jaundice, hepatocellular jaundice and in healthy volunteers. Plasma glucose levels were determined before and at 15, 30 and 45 min after glucagon. The glucose response to glucagon differed markedly in the two patient groups with significantly higher plasma glucose values in the obstructive ...

Insulin, pancreatic polypeptide, glucagon, oxyntomodulin, and two distinct glucagon-like peptides were isolated from acidic ethanol extracts of bullfrog pancreas by gel filtration followed by high pressure liquid chromatography. The amino acid sequences of pancreatic polypeptide, oxyntomodulin, and both glucagon-like peptides were determined. Frog pancreatic polypeptide contains 36 amino acid r...

A B S T R A C T Five patients with glucagonomas had elevated plasma levels of total glucagon immunoreactivity. Gel filtrations of these plasma samples on Bio-Gel P30 columns showed that most of the immunoreactivity eluted in the 3,500-(true glucagon) and 9,000-dalton fractions. After the administration of alpha cell effectors, changes in total glucagon immunoreactivity were seen which were acco...

OBJECTIVE α-cells are the second most prominent cell type in pancreatic islets and are responsible for producing glucagon to increase plasma glucose levels in times of fasting. α-cell dysfunction and inappropriate glucagon secretion occur in both type 1 and type 2 diabetes. Thus, there is growing interest in studying both normal function and pathophysiology of α-cells. However, tools to target ...

The effect of intrajugular injections of insulin, glucagon and propionate, administered singly and in combination, as possible peripheral feedbacks in the control of feed intake in wethers was studied. A complete mixed diet (25% chopped hay: 75% cracked corn) was fed ad libitum. The treatments were saline, 6 mU insulin/kg body weight (BW), 9 ng glucagon/kg BW and 1.3 mg propionate/kg BW. In Exp...