G proteins were identified in rat parotid plasma membrane-enriched fractions and in two populations of isolated secretory granule membrane fractions. Both [32P]ADP-ribosylation analysis with bacterial toxins and immunoblot analysis with crude and affinity-purified antisera specific for alpha subunits of G proteins were utilized. Pertussis toxin catalysed the ADP-ribosylation of a 41 kDa substra...

The interpretation of experimental observations of the dependence of membrane protein function on the properties of the lipid membrane environment calls for a consideration of the energy cost of protein-bilayer interactions, including the protein-bilayer hydrophobic mismatch. We present a novel (to our knowledge) multiscale computational approach for quantifying the hydrophobic mismatch-driven ...

the purpose of this study was to determine the effects of different concentrations of vitamin e (vit. e) in the quality of preserved stallion semen at 5ᵒc for a period of 48 h. semen was collected and most (≥90%) seminal plasma removed by centrifugation at 600 × g. pooled samples divided in four equal parts and diluted with extenders that was supplemented with different concentrations of vit. e...

The effects of ethanol on squid giant axons were studied by means of the sucrose-gap technique. The membrane action potential height is moderately reduced and the duration sometimes shortened by ethanol in sea water. Voltage clamp experiments showed that ethanol in sea water reduced the maximum membrane conductances for sodium (g'(Na)) and potassium (g'(K)). In experiments with multiple applica...

PURPOSE To assess the selectivity of brilliant blue G (BBG) staining by analysing the morphological components of unstained and stained tissue obtained during epiretinal membrane (ERM) removal with internal limiting membrane (ILM) peeling in BBG-assisted macular surgery. METHODS Twenty-six surgical specimens were removed from 13 eyes with epiretinal gliosis during vitrectomy using BBG for ERM...

Rift Valley fever virus (RVFV), like many other Bunyaviridae family members, is an emerging human and animal pathogen. Bunyaviruses have an outer lipid envelope bearing two glycoproteins, G(N) and G(C), required for cell entry. Bunyaviruses deliver their genome into the host-cell cytoplasm by fusing their envelope with an endosomal membrane. The molecular mechanism of this key entry step is unk...

A bstract We reconsider the supermembrane in a Minkowski background and light-cone gauge as one-dimensional theory of area preserving diffeomorphisms (APDs). Keeping membrane tension T an independent parameter we show that is proportional to coupling g this theory, such small (large) limit corresponds weak (strong) APD its SU( N ) matrix model approximation. perturbative linearization supersymm...

In this study, the influence of the salts as an additive on the performance of the membrane was investigated and an extensive work was performed to optimize PVDF hollow fiber membranes through a response surface methodology (RSM). The prepared membranes were characterized by SEM, contact angle and LEP measurement. Then, the RSM was used for the optimization of surface pore size, porosity and hy...

Background and Objectives: Red beet extract concentrate can be used as a natural colorant in food products; however, destructive effects of thermal methods to produce juice concentrate decrease nutritional value in the final product. Materials and Methods: In this study, red beet extract was concentrated using osmotic distillation method, and the best pretreatment was evaluated to increase t...