objective: bone morphogenetic protein-7 (bmp-7) is a multifunctional growth factor predominantly recognized for its osteoinductive properties. due to the high cost of this protein, the availability of bmp-7 for treatment is limited. the heterologous production of recombinant hbmp-7 has been performed in a number of expression systems. in this study a novel form of bmp-7 was expressed in eukaryo...

Synonymous codon replacement can change protein structure and function, indicating that protein structure depends on DNA sequence. During heterologous protein expression, low expression or formation of insoluble aggregates may be attributable to differences in synonymous codon usage between expression and natural hosts. This discordance may be particularly important during translation of the do...

Abstract Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), the key CO 2 -fixing enzyme in photosynthesis, is notorious for its low carboxylation. We report a highly active and assembly-competent Form II Rubisco from endosymbiont of deep-sea tubeworm Riftia pachyptila (RPE Rubisco), which shows 50.5% higher carboxylation efficiency than that high functioning Synechococcus sp. PCC7002 (7...

The maltose α-amylase AmyM from Bacillus stearothermophilus can be used for flour modification, baked goods preservation, and production. Here, we optimized the recombinant expression of in subtilis WB800 via several strategies. By screening optimal promoter, a double promoter combination (P43 PamyL) could improve level by 61.25%, compared with strong P43. Then, secretion efficiency over-expres...

Covering 2009–2022. Phenylnaphthacenoid polyketides have gained significant interest in recent years owing to their potent antibacterial and anticancer activities. Notably, more than 100 members of this class natural products been discovered from various Streptomyces species by different research groups including ours over the last 13 years. This review summarizes current knowledge discovery, c...

Introduction In recent years, methods have been developed for the expression of immunoglobulin-encoding genes in a wide range of heterologous organisms, including non-lymphoid mammalian cells, insect cells, yeast, bacteria and plants. The isolation of antibodyencoding genes from hybridoma cells and their expression in these heterologous hosts has led to a revolution in monoclonal antibody techn...

hepatitis c virus (hcv) is an important agent causing chronic liver infection, which often leads to liver cirrhosis and lethal hepatocellular carcinoma (hcc). at present, there is no effective hcv vaccine for prevention of hepatic disease and the standard treatment is neither economical nor fully effective in all the patients. however, vaccination based on structural and nonstructural proteins ...

Avian infectious bronchitis virus (IBV) defective RNAs (D-RNAs) have been used for the expression of heterologous genes in a helper-virus-dependent expression system. The heterologous genes were expressed under the control of an IBV transcription-associated sequence (TAS) derived from gene 5 of IBV Beaudette. However, coronavirus D-RNA expression vectors display an inherent instability followin...

Decreased connexin gene expression and loss of the capacity for either homologous or heterologous intercellular communication has been associated with neoplastic transformation. We tested the hypothesis that loss of gap junctional intercellular communication (GJIC) correlates with tumorigenic potential in the HeLa x skin fibroblast human hybrid cell system. Connexin gene expression, gap junctio...