Urine and serum samples from 89 healthy volunteers and three healthy individuals who underwent rubella vaccination were tested for immunoglobulin G (IgG), IgA, and IgM to rubella virus (RV) by enzyme-linked immunosorbent assay methods. Subjects with positive (n = 68) or negative (n = 21) results for serum IgG were exactly the same as those with the corresponding results for urinary IgG. Both ur...

The aim of this study was to compare ongoing production of anti-C1q antibodies (anti-C1q) in peripheral blood with serum anti-C1q levels in patients with systemic lupus erythematosus (SLE), especially in patients with nephritis. Using the ELISPOT technique for the detection of IgG and IgA anti-C1q production, 21 patients with active SLE were investigated. ELISAs for IgG and IgA anti-C1q were co...

PURPOSE This study evaluated the prevalence of ocular toxocariasis (OT) in patients with uveitis of unknown etiology who visited a tertiary hospital in South Korea and assessed the success of serum anti-Toxocara immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) as a diagnostic test for OT. METHODS The records of consecutive patients with intraocular inflammation of unknown etio...

background: helicobacter pylori strains have two classical virulence genes, the cytotoxinassociated a ( caga ) gene and the vacuolating cytotoxin a ( vaca ) gene, which are located in the cag pathogenicity island ( cag pai). serum immunoglobulin g (igg) antibodies to h. pylori , especially, the caga antigen may be a reliable marker for selection of dyspeptic patients for upper endoscopy. method...

Background: IgA serum autoantibodies against tissue transglutaminase (tTG) have an established diagnostic value in coeliac disease, and high efficacy tests are widely available for their detection. However, serological evaluation of IgA deficient subjects is still difficult. Aims: To evaluate the diagnostic potential of IgG class anti-tTG autoantibodies measured quantitatively using an enzyme l...

Anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis is a potentially fatal autoimmune syndrome in which IgG antibodies target the NR1 subunit of NMDA resulting profound dysregulation and neurotransmission.

Antibodies are essential tools of biomedical and biochemical researches. Polyclonal antibodies are produced against different epitopes of antigens. Purified F(ab')2 can be used for animal’s immunization to produce polyclonal antibodies. Human immunoglobulin G (IgG) was purified by ion exchange chromatography method. In all stages verification method of the purified antibodies was sod...

Background and Aims: Hepatitis E virus is a public health concern and about a third of people in the world live in endemic areas. Although HEV is believed to be transmitted by fecal-oral routes between humans in low sanitary conditions, but evidence of transmission have been reported in hemodialysis (HD) patients. Materials and Methods: Aim of present study was to determine the prevalence of a...

Abstract The SARS-CoV-2 pandemic has accelerated vaccine development and testing, an important step in its eradication. Health workers were included among the first subjects to be vaccinated, therefore it was necessary check their immunological status after second dose of vaccine. Serum samples belonging 24 health undergoing vaccination for (Pfizer-BioNTech) analysed: 2 them also possible obtai...