Juniperus drupacea Labill. (Cupressaceae) is a species with ecological and medicinal value. In Europe, it native only in southern Greece, listed as endangered. Due to its uniqueness, this study attempted, for the first time, an vitro propagation effort of Syrian juniper. Explants lateral shoot tips were surface-sterilized cultured on Murashige Skoog (MS) medium. The cultures subcultured MS, woo...

Ex vitro strawberry plantlets from micropropagation and coinoculated with arbuscular mycorrhizal fungi (AMF) biochar can provide beneficial health effects. In the present study, we evaluated effects of different proportions in presence absence AMF on production secondary metabolites leaves roots during ex acclimatization. Additionally, enzymatic activity substrate enriched was analyzed. The exp...

Background: Micropropagation, an in vitro vegetative propagation technique using small propagules is one of the main applications plant tissue culture. It can be used to clone specific plants with desired traits and reduce cost propagation. In this study, we developed a protocol for micropropagation Eucalyptus microcorys F.Muell selected mature tree, which tested various combinations different ...

Leaf explants of kaladium dwubarwne Caladium bicolor (Aiton) Vent., were cultured in vitro on MS media supplemented with 25 different concentrations of BAP and NAA in order to determine the appropriate concentrations for micropropagation. All combinations induced callus formation on explants. Callus production on leaves explants grown on control medium was very low. The medium enriched with 4 m...

The establishment of new in vitro cultures is often a difficult task due to low growth of initial explants. The explant has to adapt to the new nutritional and environmental conditions, in addition to the surface-disinfection process. Since the explant origin plays an important role, in this work, the effect of the origin of the explants (micropropagated or conventionally propagated plants) in ...

In vitro regeneration ability of four varieties potato (Solanum tuberosum) was evaluated for plantlet production applying hormonal concentrations and combinations. Shoot root characteristics were observed at 15 days after inoculation sprout explants. There significant variation found among the treatments in studied traits. The genotype Chollisha exhibited superior performance genotypes. Out tea...

Nidularium minutum is an ornamental bromeliad from the Brazilian Rainforest. The micropropagation of this species essential for obtaining plants available conservation programs or commercial use. Our study aimed to establish efficient plant production method by in vitro sprouting. This takes a long time sprout vitro, and 10% produce shoots culture medium without growth regulators (PGRs). When s...

In this study, the efficient micropropagation protocol of Astragalus mongholicus Bunge was established and also triterpenoid saponin polysaccharide content in ethanol, methanol aqueous extracts different samples were determined by using spectrophotometric methods to investigate whether biologically active compounds depends on stage development plant during vitro culture. The total saponins poly...