Introduction: Alpha-Momorcharin (α-MMC) is a member of the ribosome-inactivating protein (RIP) family that has been widely used as an antitumor, antiviral and antifungal agent. Methods: In this study, codons DNA encoding α-MMC were optimized for expression in E. coli cloned into pET-28a(+) vector. The was then expressed strain BL21 (DE3) purified by nickel affinity chromatography. Results: Unde...

the µ (mu) opioid receptors, which mediate the effects of morphine, are widely distributed in brain. the purpose of this study was to design a simple expression system for rat µ-receptor in escherichia coli (bl21). in this laboratory study, rat µ-receptor cdna was isolated from pcdna3 vector using xba1 and hind3 restriction enzymes. pet-15b was digested by nco1 restriction enzyme. µ-receptor cd...

The realization that the synthesis of proteins is often under the control of repressorsl' 2 has posed a central question in molecular biology: What is the nature of the controlling substances? The scheme of negative control proposed by Jacob and Mionod envisages that certain genes, regulatory genes, make products that can act through the cytoplasm to prevent-the functioning of other genes. Thes...

Different bacteria-derived systems for regulatable gene expression have been developed for the use in mammalian cells and some were also successfully adopted for in vivo use in vertebrate model organisms. However, certain limitations apply to most of these systems, including leakiness of transgene expression, inefficient transgene silencing or activation, as well as limited tissue accessibility...

Most bacteria possess peptidoglycan (PG), an extracytoplasmic polymer that protects them from osmotic lysis and that serves as an anchor for cellular components, including virulence factors (10). PG biogenesis is the target of many antibiotics, but increasing resistance is rendering many such antibiotics ineffective (6). Recently, MurJ (formerly MviN) has been shown essential for PG biogenesis ...

The gene that makes the lac repressor functions at an extremely low rate. It synthesizes only a few thousandths of a per cent of the total protein of an E. coli cell: about 5-10 molecules each generation.1 In order to study this molecule more easily, we have sought to enhance the amount present in a cell. Two approaches have been successful. The repressor gene, the i gene, itself can be mutated...

We have developed a technique to establish catalogues of protein products of arrayed cDNA clones identified by DNA hybridisation or sequencing. A human fetal brain cDNA library was directionally cloned in a bacterial vector that allows IPTG-inducible expression of His6-tagged fusion proteins. Using robot technology, the library was arrayed in microtitre plates and gridded onto high-density in s...

  Objective(s): Streptokinase (SKa) is an antigenic protein which is secreted by Streptococcus pyogenes. Streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. In the present study, recombinant streptokinase from S. pyogenes was produced and showed that recombinant SKa protein was recognized by infected human sera using Western blot analy...