BACKGROUND Diverse microbiota exist in the lower respiratory tract. Although next generation sequencing (NGS) is the most widely used microbiome analysis technique, it is difficult to implement NGS in clinical microbiology laboratories. Therefore, we evaluated the performance of conventional culture methods together with matrix-assisted laser desorption/ionization time-of-flight mass spectromet...

Considerable advances have been made in the genetics and molecular biology of lactic acid bacteria, including Lactococcus, Lactobacillus, Leuconostoc, Pediococcus and Streptococcus spp. These have resulted in the construction of constitutive gene expression cassettes, inducible gene expression systems, and specific protein targeting systems for these bacteria. These developments are important i...

in this study, the essential oil (eo) of pistacia atlantica subsp. kurdica was added at the rate of 10 g/kg of daily diet of rainbow trout and its effect on gut microbiota (enterobacteriaceae, lactobacillus spp., total count), growth performance and antioxidant status of rainbow trout fillet was investigated. sixty apparently healthy rainbow trout with an approximate weight of 150 g ±3.5 were r...

The biodiversity of Lactobacillus spp. in colostrum samples from 116 Chilean mothers was analyzed by PCR and 16S rDNA sequencing. Lactobacilli were isolated in 55.3% of the samples, with concentrations of 3.33 +/- 0.55 (log CFU/ml). The predominant species were L. plantarum (64%), L. fermentum (16%) and L. pentosus (9%). 28% of the isolated strains were resistant to gastric pH and bile salts, s...

The analysis of semen microbiota is difficult due to the lack established criteria for interpretation microbiological tests. aim study was determine stable clusters analyzed by real-time PCR in samples with normozoospermia. Semen 227 men normal spermiograms were included study. quantity total bacterial DNA and at least one group microorganisms more than 103 GE/ml 107 (41.7%) samples. Four preva...

PepV, a dipeptidase found in culture fluids of Streptococcus gordonii FSS2, was purified and characterized, and its gene was cloned. PepV is a monomeric metalloenzyme of approximately 55 kDa that preferentially degrades hydrophobic dipeptides. The gene encodes a polypeptide of 467 amino acids, with a theoretical molecular mass of 51,114 Da and a calculated pI of 4.8. The S. gordonii PepV gene i...

Exopolysaccharides as well as other bioactive substances of Lactic acid bacteria (LAB) attracted much attention due to its specific structure and functions, e.g. tolerance to gastrointestinal (GI) stress, adhesion on the intestinal mucosa, inhibition against pathogens, and modulation of immune system. In this mini-review, we have summarized the recent progress of exopolysaccharides of Lactobaci...

Aims: To determine and compare the antimicrobial activity of chewing stick Salvadora persica (Miswak), dental powder toothpastes sold in Umuahia, Abia state Nigeria against selected oral bacteria (Streptococcus spp, Staphylococcus Porphyromona spp Lactobacillus spp). Method: The (Miswak) extracts (ethanol aqueous), two (Agnes nwamma N-sol) five brand (Close-Up, Oral-B, Colgate, MacClean Pepsode...

When undergoing chemotherapy, it is easy for patients to develop an intraoral disorder as an adverse event. If intraoral disorder worsens, we cannot but stop chemotherapy. In this situation, oral bacteria are often detected in the blood. The goal of perioperative oral function management (POMF) during chemotherapy is to allow chemotherapy to proceed smoothly while maintaining the intraoral envi...

Background and Objective: Lactobacilli are known for their probiotic activities and potential to combat pathogens. This study aimed to investigate the antimicrobial activity of Lactobacillus strains isolated from various sources against pathogenic bacteria, with the objective of identifying potential bacteriocin producers. Methods: A total of 140 samples were collected from different sources. ...