Objective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-G...

in vitro maturation (ivm) is emerging as a popular technology at the forefront of fertility treatment and preservation. however, standard in vitro culture conditions usually increase reactive oxygen species (ros), which have been implicated as one of the major causes for reduced embryonic developmental competence. it is well-known that higher than physiological levels of ros triggers granulosa ...

Mammalian cell culture media used for the manufacture of therapeutic protein have advanced systematically from serum-containing into animal-free, protein-free and chemically defined formulations over the past decades. Initially driven by patient safety concerns associated with the use of animal-derived medium components, and later by inconsistent cell culture performance due to variability in p...

Despite considerable progress in the development of cell culture techniques, including the development of the serum- and protein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum (FBS) supplemented media are still commonly used: a practice that raises ethical, scientific and safety concerns. The use of FBS in hybridoma culture media is examined here, ...

Metabolic oligosaccharide engineering has been employed to introduce fluorine-containing groups onto mammalian cell surfaces. Incubation of HeLa, Jurkat, and HL60 cells in culture with fluorinated sialic acid and mannosamine analogues resulted in cell-surface presentation of fluorinated glycans. Metabolic conversion of fluorinated precursors was detected and quantified by DMB-derivatization and...

For successful cell culture in microfluidic devices, precise control of the microenvironment, including gas transfer between the cells and the surrounding medium, is exceptionally important. The work is motivated by a polydimethylsiloxane (PDMS) microfluidic oxygenator chip for mammalian cell culture suggesting that the speed of the oxygen transfer may vary depending on the thickness of a PDMS ...

Over the last century, mammalian embryos have been used extensively as a common animal model to investigate fundamental questions in the field of developmental biology. More recently, the establishment of transgenic and gene-targeting systems in laboratory mice has enabled researchers to unveil the genetic mechanisms underlying complex developmental processes (Mak, 2007). However, our understan...

Background: While it is possible to routinely cryopreserve embryos from several mammalian species, the cryopreservation of embryos has largely been limited by their high sensitivity to chilling injury. Many factors such as the stage of embryonic development, cryoprotectant toxicity, the composition of the vitrification solution and cooling and warming rates can influence survival of embryos aft...

background: chlamydia trachomatis is the most reported bacterial sexually transmitted disease, especially among young women worldwide. aim of this study was comparison to evaluate the prevalence of chlamydia trachomatis infection in woman with tubal infertility by means of pcr and cell culture techniques. methods: fifty-one women with confirmed tfi were enrolled in this study in (avicenna infer...

Recent advances in mammalian, insect, and stem cell cultivation and scale-up have created tremendous opportunities for new therapeutics and personalized medicine innovations. However, translating these advances into therapeutic applications will require in vitro systems that allow for robust, flexible, and cost effective bioreactor systems. There are several bioreactor systems currently utilize...