Biofilms as a form of adaptation are beneficial for bacterial survival and may be hot spots horizontal gene transfer, including conjugation. The aim this research was to characterize the biofilm biomass, viable cell ratios conjugative transfer pOX38 plasmid, an F-plasmid derivative, from Escherichia coli N4i strain (donor) into uropathogenic E. DL82 (recipient) within dual-species biofilms with...

Abstract Background The discovery of the superbug mcr-1 -positive Escherichia coli (MCRPEC) has drew greet attention. Swine-origin multi-drug resistant MCRPEC been a potential threat to public health and safety. However, there were few detailed studies have reported on swine in Guangxi, South China. Results In this study, thirty-three strains detected from 142 E. 116 samples Guangxi 2018. Which...

Plasmid pPL1010 is a 7.0-kilobase derivative of plasmid pUB110 that harbors the cohesive end site of the bacteriophage SP02 genome. Plasmid pPL1017 is a 6.8-kilobase derivative of plasmid pC194 that contains the immunity region of bacteriophage phi 105 and the cohesive end site of bacteriophage SP02. These plasmids are transducible by bacteriophage SP02 at a frequency of 10(-2) transductants pe...

toxoplasmosis is a worldwide infection which is commonly asymptomatic but can cause serious medical problems in immunocompromised individuals and fetus. the infection also causes considerable economic loss because of abortion in livestock, mostly in sheep and goats. dna vaccination may be a powerful approach against intracellular parasites such as toxoplasma gondii. the goal of this study was t...

Abstract In April 2019, hydroponically cultivated cucumber plants with characteristic symptoms of crazy root disease were found in two different commercial production greenhouses Poland. Due to excessive and inappropriate growth, this led a reduction yield deterioration the general conditions infected plants. Bacteria isolated from roots subjected morphological evaluation, as well molecular, bi...

Plasmid toxins CcdB and ParE are part of addiction systems promoting plasmid maintenance. Both target host DNA gyrase, as do quinolones and plasmid-determined Qnr proteins that protect gyrase from quinolone inhibition. We cloned qnrB4, qnrS1, ccdB, parE, and the antitoxin-encoding genes ccdA and parD on compatible plasmids and tested them in combination. CcdB and ParE had no specific effect on ...

introduction: resistance of pathogenic organisms to countenance antibiotics has become a worldwide problem with serious consequences on the treatment of infectious diseases. the aim of this study was to evaluate antibiotic resistance and also the detection of transferred antibiotic resistance by plasmid in clinical proteus isolates. materials and methods: a total of 250 urine samples were colle...

background: alpinia galanga l. belonging to the family zingiberaceae is widely grown in the state of kerala, india. they are effective antioxidant and free radical scavenger under both in vitro and in vivo condition. the efficacy of the isolated flavonoids in conferring protection from radiation induced damages to genomic dna was studied. materials and methods: the bioflavonoids, galangin and k...