Previously, we reported that the GABA(A) receptor antagonist picrotoxin also antagonizes serotonin (5-HT)3 receptors and that its effects are subunit-dependent. Here, we sought to identify amino acids involved in picrotoxin inhibition of 5-HT3 receptors. Mutation of serine to alanine at the transmembrane domain 2 (TM2) 2' position did not affect picrotoxin (PTX) sensitivity in murine 5-HT3A rec...

system (1). The studies of Killam (2) and Killam and Bain (3), whieh used convulsant hydrazides and demonstrated thatr GABA has an inhibitory function in the CNS, suggested a role for GABA in neurotoxicology. There are two types of GABA receptors, GABIVA and GABAB, The GABAA receptor is a membrane bound eomplex consisting of at least three binding sites for GABA, benzodiazepines, the barbiturat...

Germ-free mice were found to be more sensitive than conventional mice to poisoning by hemicholinium-3, hexamethonium chloride, strychnine sulfate, sodium pentobarbital, sodium barbital, and histamine diphosphate. On the other hand, conventional mice were more sensitive to atropine. No difference between germ-free and conventional mice was found in sensitivity to d-tubocurarine, metrazol, and pi...

Articles in PresS. J Neurophysiol (December 17, 2008). doi:10.1152/jn.90279.2008 Copyright © 2008 by the American Physiological Society.