The purpose of this study was to compare hybrid capture assay with PCRs using different primers for the L1, E6-E7 regions for the detection of human papillomavirus (HPV) genome. One hundred twenty-five cervical smears with normal (n=42) and abnormal (n=83) cytology were investigated. Those at high-risk for HPV were studied by hybridization antibody capture assay and PCR with the pU-1M/pU-2R pri...

We have developed a strategy for multiplex PCR based on PCR suppression. PCR suppression allows DNA target amplification with only one sequence-specific primer per target and a second primer that is common for all targets. Therefore, an n-plex PCR would require only n + 1 primers. We have demonstrated uniform, efficient amplification of targeted sequences in 14-plex PCR. The high specificity of...

Background: The pathogenesis of migraine involves immune-mediated mechanisms in the vascular endothelium. Toll like receptor 4 (TLR-4) is a signaling receptor of innate immunity which plays a role in various neuropathologies related to neuron inflammation. Objective: This case/control study is aimed to investigate whether TLR- 4 896A/G variation is related to migraine headaches in an Iranian po...

http://bfw.ac.at/200/1859.html The data collected: A data base is presented which collects published primer information for chloroplast DNA. Additional primers were designed in order to fill gaps were no or little primer information could be found. Chloroplast genes evolve slowly, and many primers have been designed to work across species ('universal primers', e.g. Taberlet et al. 1991, Grivet ...

The simultaneous extraction of plant organelle (mitochondria and plastid) genes during the DNA extraction step is a major limitation in investigating the community structures of bacteria associated with plants because organelle SSU rRNA genes are easily amplified by PCR using primer sets that are specific to bacteria. To inhibit the amplification of organelle genes, the locked nucleic acid (LNA...

The development of vectors such as P1, P1-derived artificial chromosome (PAC) or bacterial artificial chromosome (BAC) (2,6,8) has permitted the cloning of large DNA fragments. These vectors have become common in largescale sequencing and mapping projects. Therefore, a reliable primer walking protocol for P1 plasmids is desirable. It has been demonstrated previously that sequencing of P1 plasmi...

One of the limitations of PCR to analyse a region of interest is that it requires knowledge of the flanking sequences in order to design amplification primers. Several adaptations of PCR have been described which allow some unknown sequences adjacent to regions of known sequence to be amplified. Primers directed to repetitive sequences, for instance Alu repeats can be used to pair with a primer...

mouse genotyping by the three-primer multiple PCR. However, both of the smaller PCR products of 1068 (specific for the wild-type allele of mGluR4) and 1170 bp (specific for the mGluR4 transgene) were visible. Thus, all three allele combinations were clearly distinguishable on agarose gels stained with ethidium bromide. DNA from wildtype mice give a single smaller band (1068 bp; see Figure 2B, l...

We have developed statistical models for estimating the failure rate of polymerase chain reaction (PCR) primers using 236 primer sequence-related factors. The model involved 1314 primer pairs and is based on more than 80 000 PCR experiments. We found that the most important factor in determining PCR failure is the number of predicted primer-binding sites in the genomic DNA. We also compared dif...

The genetic diversity of 33 Nigerian Helicobacter pylori isolates were studied using RAPD, PCR-RFLP and Southern blot analysis of ureA or ureCD gene probes. RAPD was able to distinguish the following number of isolates using the primers 3880: 5'-AAGAGCCCGT-3' (28), 3881 :5'-AACGCGCAAC-3' (33) and OPH8 :5'-GAAACACCCC-3' (25). Southern blot analysis using the ureCD probe was also able to distingu...