نتایج جستجو برای: primer arms pcr technique
تعداد نتایج: 817030 فیلتر نتایج به سال:
background and objective: mmp enzymes are a family of membrane proteins that are capable of digesting extracellular matrix compounds (ecm) and basement membrane. matrilysin enzyme is the smallest member of mmp family that is encoded by mmp-7 gene (matrilysin). according to the reports, g allele of -181 a/g single nucleotide polymorphism of mmp-7 gene causes an increase in the expression of this...
سرطان کولورکتال شامل سرطان در ناحیه ی کولون، رکتوم و زائده ی آپاندیس است. سرطان کولون و رکتوم خصوصیات مشترک زیادی با یکدیگر داشته، به همین دلیل اغلب آنها را با هم در نظر گرفته و به عنوان سرطان کولورکتال شناسایی می-شوند. در ایران سالیانه حدود 2262 نفر در اثر ابتلا به این سرطان جان خود را از دست می دهند. سرطان ابتدا به صورت پولیپ-های خوش خیم ظاهر می گردد که می تواند به سرطان تهاجمی و کشنده تبدیل ...
Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genotyping. One such method is reported here, named tetra-primer ARMS-PCR, which employs two primer pa...
Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given...
New, quick, and inexpensive methods for genotyping novel caprine Fec gene polymorphisms through tetra-primer ARMS PCR were developed in the present investigation. Single nucleotide polymorphism (SNP) genotyping needs to be attempted to establish association between the identified mutations and traits of economic importance. In the current study, we have successfully genotyped three new SNPs ide...
زمینه و هدف: تالاسمی اینترمدیا یک بیماری اتوزوم مغلوب بوده که از نظر بالینی طیفی بسیار گسترده از اختلالات وابسته به هموگلوبین را شامل می شود و از نظر شدت بیماری بین تالاسمی ماژور و مینور قرار میگیرد. سطح بالای هموگلوبین جنینی تاثیر عمدهای بر وخامت کلینیکی (بالینی) این بیماری دارد، به طوری که افزایش تولید hbf شدت بیماری را کاهش میدهد. عوامل مختلفی درون لوکوس بتا گلوبین میتوانند در کاهش شدت ...
Background: The transversion of G to T (G894T) in human endothelial nitric oxide synthase (eNOS) gene has profound effects such as male infertility, recurrent miscarriage, multiple sclerosis and cardiovascular diseases.Objectives: Development of a new Multiplex Tetra-Primer Amplifi cation Refractory Mutation System - Polymerase Chain Reaction (T-ARMS-PCR) for detection of...
Kappa-casein (κ-casein) is one of the most abundant milk proteins. Its main function is to avoid the aggregation of casein micelles, keeping them, and therefore calcium phosphate, in pockets in solution. In bovines, a κ-casein functional polymorphism has been associated with fat, calcium, and protein milk contents and faster curd contraction in cheese production. Quicker curd contraction reduce...
BACKGROUND The tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) is a fast and economical means of assaying SNP's, requiring only PCR amplification and subsequent electrophoresis for the determination of genotypes. To improve the throughput and efficiency of T-ARMS-PCR, we combined T-ARMS-PCR with a chimeric primer-based temperature switch PCR (TSP) strategy, and used capil...
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