Eukaryotic genes generate multiple RNA transcript isoforms though alternative transcription, splicing, and polyadenylation. However, the relationship between human transcript diversity and protein production is complex as each isoform can be translated differently. We fractionated a polysome profile and reconstructed transcript isoforms from each fraction, which we term Transcript Isoforms in P...

Specific isoforms of myofibrillar proteins are expressed in different muscles and in various fiber types within a single muscle. We have isolated and characterized monoclonal antibodies against C-proteins from slow tonic (anterior latissimus dorsi, ALD) and fast twitch (pectoralis major) muscles of the chicken. Although the antibody against "fast" C-protein (MF-1) did not bind to the "slow" iso...

DNA transposons are exposed to a molecular domestication process, which results in the formation of neogenes,which may play role human genetic instability. TIGD3 (Tigger-derived [TIGD] family proteins) is one ofthese Neogene, and its genome unknown.Aim: The expression Neogene colorectal cancer cell lines putative function carcinogenesisare being investigated.Method: protein gene was investigate...

Fas/APO-1 and p55 tumor necrosis factor (TNF) receptor (p55-R) activate cellular mechanisms that result in cell death. Upon activation of these receptors, Fas/APO-1 binds a protein called MORT1 (or FADD) and p55-R binds a protein called TRADD. MORT1 and TRADD can also bind to each other. We have cloned a novel protein, MACH, that binds to MORT1. This protein exists in multiple isoforms, some of...

We have mapped the expression of the tonoplast intrinsic protein (TIP) gene family members in Arabidopsis seeds by fluorescent protein tagging of their genomic sequences and confocal microscopy. Three isoforms (TIP1;1, TIP2;1, and TIP2;2) have distinct patterns of expression in maternal tissues (outer integument and placento-chalazal region). Two isoforms, TIP3;1 and the previously uncharacteri...

Tau is a microtubule-binding protein expressed in neurons and the equal ratio between 4-repeat (4R) 3-repeat (3R) isoforms are maintained normal adult brain function. Dysregulation of 3R;4R causes tauopathy human that recapitulate tau health disease will provide platform for elucidating pathogenic processes involving pathology. We carried out extensive characterizations derived by microRNA-indu...