Background: The major therapeutic failure in clinical settings is due to problematic biofilm-producingbacteria like Pseudomonas aeruginosa. This study aims investigate the effect of dual-species biofilms,monosaccharide, and D-amino acids on pseudomonal biofilm. Methodology: A total 130 patients withcatheter-associated urinary tract wound infections were involved this study. Quantitative biofilm...

Two isolates, one from the genus Pseudomonas and the second from Citrobacter, were isolated from a wound dressing-associated biofilm. Following whole-genome sequencing, the two isolates presented genes encoding for resistance to antibiotics and those involved in exopolysaccharide production.

Cyclic diguanylate (c-di-GMP) affects bacterial physiological and biochemical functions like biofilm, motility, virulence, secretion systems. GGDEF/EAL-domain proteins, participating in c-di-GMP synthesis degradation, are widely present Pseudomonas, with various structures functions. Pseudomonas glycinae MS82 is a rhizosphere bacterium that protects mushroom against the pathogenic fungi. Althou...

Biofilms have been implicated as an important reservoir for pathogens and commensal enteric bacteria such as Escherichia coli in natural and engineered water systems. However, the processes that regulate the survival of E. coli in aquatic biofilms have not been thoroughly studied. We examined the effects of hydrodynamic shear and nutrient concentrations on E. coli colonization of pre-establishe...

P. aeruginosa is an opportunistic pathogenic bacterium responsible for both acute and chronic infections. Beyond its natural resistance to many drugs, its ability to form biofilm, a complex biological system, renders ineffective the clearance by immune defense systems and antibiotherapy. The objective of this report is to provide an overview (i) on P. aeruginosa biofilm lifestyle cycle, (ii) on...

Nutrient concentration profiles are affected by the mass transport outside the biofilm, inside the biofilm, and by the structure of the biofilm. To understand the distribution of biofilm activity, it is necessary to correlate the local nutrient concentration, local mass transport in the biofilm and the biofilm structure. The correlations among surface averaged dissolved oxygen concentration, su...

Pseudomonas aeruginosa PAO1 produces three polysaccharides, alginate, Psl, and Pel that play distinct roles in attachment and biofilm formation for monospecies biofilms. Considerably less is known about their role in the development of mixed species biofilm communities. This study has investigated the roles of alginate, Psl, and Pel during biofilm formation of P. aeruginosa in a defined and exp...

AIMS To investigate the dynamics of binary culture biofilm formation through use of both the Sorbarod model of biofilm growth and the constant depth film fermenter (CDFF). METHODS AND RESULTS Pseudo steady-state biofilm cultures of laboratory and clinical strains of Pseudomonas aeruginosa, selected on the basis of their ability to produce a Burkholderia cepacia growth-inhibitory substance, we...

Department of Molecular Microbiology and Biotechnology, Institute Biology Leiden, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands 2Department of Pharmaceutical Biology, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara, Yogyakarta 55281, Indonesia Koppert Biological Systems, Veilingweg 14 2650 AD Berkel en Rodenrijs, The Netherlands. Department of Pharmacy, Faculty of M...

Objective(s):The inhibitory effects of iron chelators, and FeCl3 chelation on biofilm formation and swarming motility were investigated against an opportunistic human pathogen Pseudomonas aeruginosa. Materials and Methods:The inhibitory activity of 2,2’-bipyridyl, lipoic acid, kojic acid and picolinic acidonbiofilm formation of P. aeruginosa strain PAO1 and three clinical isolates (P. aeruginos...