Intracellular invasion of professional phagocytic cells like monocytes and macrophages by a pathogen usually triggers the apoptosis of the host cells. The aim of this study was to evaluate if Pseudomonas aeruginosa, although not considered a classic intracellular pathogen, could adhere to endothelial cell surface, invade the intracellular compartment and subsequently induce apoptosis of the cel...

4 Department of International Health, Immunology and Microbiology, Faculty of Health and 5 Medical Sciences, University of Copenhagen, Copenhagen, Denmark. 6 Department of Microbiology, University of Washington, Seattle, WA 98195-7242, USA. 7 Medicinal Chemistry Research, Lundbeck Research Denmark, H. Lundbeck A/S, Ottiliavej 9, DK8 2500 Valby, Denmark. 9 Department of Chemistry, Technical Univ...

The human pathogen Pseudomonas aeruginosa uses the pqs quorum sensing system to coordinate the production of its broad spectrum of virulence factors to facilitate colonization and infection of its host. Hereby, the enzyme PqsD is a virulence related quorum sensing signal synthase that catalyzes the central step in the biosynthesis of the Pseudomonas quinolone signals HHQ and PQS. We developed a...

Bacteria that survive under variable conditions possess an assortment of genetic regulators to meet these challenges. The group IV or extracytoplasmic function (ECF) sigma factors regulate gene expression in response to specific environmental signals by altering the promoter specificity of RNA polymerase. We have undertaken a study of PvdS, a group IV sigma factor encoded by Pseudomonas syringa...

The interaction between propranolol (PROP) and human serum albumin (HSA) was studied in the presence of dimethyl methylphosphonate (DMMP). DMMP is usually considered as a simulant for chemical warfare agents (CWAs). For this purpose fluorescence quenching, resonance light scattering (RLS), synchronous, three-dimensional fluorescence spectroscopy and molecular dynamics (MD) simulation were emplo...

This study investigated the effect of external resistance (Rext) on dynamic evolution microbial communities in anodic biofilms single-chamber fuel cells fueled with acetate and inoculated municipal wastewater. Anodic developed under different Rext (0, 330 1000 ohms, open circuit condition) were characterized as a function time during two weeks growth using 16S rRNA gene sequencing, cyclic volta...

As a result of the looming antimicrobial resistance crisis, there is an urgent need for novel treatments. This particularly true hard-to-treat Gram-negative bacteria, as many agents are unable to cross cell membrane gain access interior, and thus elicit therapeutic response. Herein, evidence provided use anionic supramolecular self-associating amphiphiles (SSAs) efficacy enhancers commonly used...