In this study, our previously reported novel synthetic gene encoding 166 residues of interferon-? was used for an efficient expression of IFN-?. The synthetic gene was cloned into pET21a expression vector and transferred into E. coli. Recombinant protein was over-expressed in the E. coli. Identity of the recombinant protein was confirmed by western blot analysis. The recombinant protein was bio...

diphtheria is a fatal disease caused by exotoxin of corynebacterium     diphtheria .  this toxin consists of   two chains, catalytic chain (a) and binding (b) chain. by binding chain (b),   the toxin binds to its receptor on numerous body cells such as myocardial,   kidney and peripheral nerve cells. after entering, catalytic chain (a)   inhibits protein synthesis and finally can cause cell dea...

b ackground: the prevalence of urinary tract infection (uti) is really high in the world. escherichia coli is a major agent of uti. one of the strategies for decreasing uti infections is vaccine development. as the attachment is a really important stage in colonization and infection, at- tachment inhibition has an applied strategy.  fimh protein is a major factor during bacterial colonization i...

Crystalline arrays of proteinaceous subunits forming surface layers (S-layers) are now recognized as one of the most common outermost cell envelope components of prokaryotic organisms. The surface layer protein of Lactobacillus acidophilus ATCC4356 is composed of a single species of protein of apparent molecular weight of 43-46 KDa. Considering the Lactobacillus acidophilus ATCC4356 having the ...

The four RNAs of brome mosaic virus induce substantial incorporation of amino acids into protein when used as messengers in a cell-free protein-synthesizing system derived from wheat embryo. RNA 4 is highly efficient as a monocistronic messenger for the viral coat protein. Acetate, derived from acetyl coenzyme A, is incorporated into the product made in vitro. Although RNA 3 also contains the c...

Alterations in the genomic position of the tobacco mosaic virus (TMV) genes encoding the 30-kDa cell-to-cell movement protein or the coat protein greatly affected their expression. Higher production of 30-kDa protein was correlated with increased proximity of the gene to the viral 3' terminus. A mutant placing the 30-kDa open reading frame 207 nucleotides nearer the 3' terminus produced at leas...

Tobamoviruses, mostly isolated from solanaceous plants, may represent ancient virus lineages that have codiverged with their hosts. Recently completed nucleotide sequences of six nonsolanaceous tobamoviruses allowed assessment of the codivergence hypothesis and support a third subgroup within tobamoviruses. The genomic sequences of 12 tobamoviruses and the partial sequences of 11 others have be...

Transient expression of proteins in plants has become a choice to facilitate recombinant protein production with its fast and easy application. On the other hand, host defensive mechanisms have been reported to reduce the efficiency of transient expression in plants. Hence, this study was designed to evaluate the effect of cap analog and Potato virus A helper component proteinase (PVA HC-Pro) o...

objective: escherichia coli o157:h7 is a gram-negative rod-shaped bacterium. e coli o157:h7 is an enterohemorrhagic (ehec) strain of the bacterium escherichia coli and a cause of foodborne illness. infection often leads to bloody diarrhea by producing a toxin called shiga toxin, which damages the intestines, and occasionally leads to kidney failure, especially in young children and elderly peop...