نتایج جستجو برای: rflp method genetic polymorphism
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Introduction: Hepatitis B virus (HBV) infection is a major health problem worldwide and may lead to serious clinical complications, including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. The host’s genetic background in immune system genes is a crucial etiologic factor in progression of HBV infection to chronic disease or clearance of the virus from the body. Interleu...
An analysis of restriction fragment length polymorphism (RFLP) using eight residential insertion sequence (IS) elements as hybridization probes reveals that the genome of resting bacteria is more dynamic than it was long believed. Escherichia coli strains stored in agar stabs for up to 30 yr accumulate a genetic variation which is correlated to time of storage. This spontaneous mutagenesis is o...
A detailed RFLP map was constructed of the distal end of the short arm of chromosome 1D of Aegilops tauschii, the diploid D-genome donor species of hexaploid wheat. Ae. tauschii was used to overcome some of the limitations commonly associated with molecular studies of wheat such as low levels of DNA polymorphism. Detection of multiple loci by most RFLP probes suggests that gene duplication even...
background: indirect genetic diagnosis using polymorphic dna markers can detect carriers of hemophilia a. this technique is preferable in developing countries because of its simplicity and cost effectiveness compared to direct mutation analysis. in the present study, we examined usefulness of intragenic marker bcl i restriction fragment length polymorphism (rflp) at intron 18, for carrier detec...
ADIPOQ gene polymorphisms were indicated to be associated with coronary artery disease (CAD) in diabetic patients, however, published studies reported inconsistent results. We performed this meta-analysis to reach a more accurate estimation of the relationship between two common ADIPOQ genetic polymorphisms (rs2241766 and rs1501299) and CAD risk in diabetic patients. Eligible studies were retri...
introduction : cytotoxic t-cell lymphocyte antigen 4 (ctla-4) is a member of the superfamily of immunoglobulins that are mainly expressed by activated t cells. it is established that blockade of ctla-4 receptors leads to the enhancement of an immune response. different polymorphisms of the ctla-4 gene have been described which cause increased susceptibility to various malignancies such as lymph...
Background and Objective: Asthma is a common respiratory disease caused by acute and chronic bronchial inflammation. Clinical manifestations of the disease are closely related to genetics. IL-4 is a cytokine of TH2 lymphocytes, polymorphism in prompter region, C-589T, is associated with IL-4 production, while IFN-γ, is a cytokine of TH1, and A+874T polymorphism in interon 1 of IFN-γ is associat...
Objective. Takayasu’s arteritis (TA) is a chronic, rare granulomatous panarteritis of unknown aetiology involving mainly the aorta and its major branches. In this study, genetic susceptibility to TA has been investigated by screening the functional single nucleotide polymorphism (SNP) of PTPN22 gene encoding the lymphoid-specific protein tyrosine phosphatase. Methods. Totally, 181 patients with...
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