1. The paper describes the development and construction of an apparatus for maintaining a normal microbial population of the rumen under strictly controlled conditions over long periods of time. 2. The apparatus is simple to construct and operate. It is possible to do four replicate experiments at the same time. 3. The results of three experiments are given. The experiments showed that when the...

The aim of this study was to evaluate the effect of fat supplementation for enhancing milk conjugated linoleic acid (CLA) in early lactation without affecting ruminal fermentation. 24 Holstein dairy cows were assigned to a completely randomized design employing a 2 × 4 factorial arrangement. Factors were particle size (15 and 25 mm) and two different sources of alfalfa supplemented with and wit...

The Effects of using Acid-Consuming Bacteria and Different Buffers with High Concentration Diets on In Vitro Digestion Fermentation Activity Anaerobic Fungi Fattening Lambs Rumen

The objective of this study was to evaluate the in vitro effects of coconut materials on ruminal methanogenesis and fermentation characteristics, in particular their effectiveness for mitigating ruminal methanogenesis. Fistulated Holstein cows were used as the donor of rumen fluid. Coconut materials were added to an in vitro fermentation incubated with rumen fluid-buffer mixture and timothy sub...

The study aimed to investigate the effects of gynosaponin on in vitro methanogenesis under different forage-concentrate ratios (F:C ratios). Experiment was conducted with two kinds of F:C ratios (F:C = 7:3 and F:C = 3:7) and gynosaponin addition (0 mg and 16 mg) in a 2×2 double factorial design. In the presence of gynosaponin, methane production and acetate concentration were significantly decr...

This study evaluated the in vitro effect of medicinal plant extracts on ruminal methanogenesis, four different groups of methanogens and ruminal fermentation characteristics. A fistulated Holstein cow was used as a donor of rumen fluid. Licorice and mugwort extracts (Glycyrrhiza uralensis and Artemisia capillaris, 0.5% and 1% of total substrate DM, respectively), previously used as folk remedie...

This study was conducted to investigate the effects of eucalyptus (E. Camaldulensis) crude oils (EuO) supplementation on voluntary feed intake and rumen fermentation characteristics in swamp buffaloes. Four rumen fistulated swamp buffaloes, body weight (BW) of 420±15.0 kg, were randomly assigned according to a 2×2 factorial arrangement in a 4×4 Latin square design. The dietary treatments were u...

Solid-state fermentation can be used to produce feeds for ruminants, which can provide an enriched population of yeasts to improve ruminal fermentation. Fermentation of apple bagasse was performed to obtain a yeast-rich product, with the objective of isolating, identifying, and characterizing yeast strains and testing their capability to enhance in vitro ruminal fermentation of fibrous feeds. Y...

Background: Fat supplementation in ruminants ration often adversely affect dry matter intake (DMI), rumen fermentation and nutrients digestion. Basal diet ingredients is an undeniable determinant of ruminants responses including performance, chewing behaviour and nutrient digestibility to fat supplementation. Objective: Current study was conducted to evaluate the effect of Fish oil (FO) supplem...