Background This paper aims to explore the application value of tuberculosis-specific enzyme-linked immunospot assay (T-SPOT.TB) in the diagnosis of tuberculosis. Methods Fifty one patients with tuberculosis (TB) admitted to Wuxi No.5 People's Hospital, Wuxi, China from June 2015 to June 2017 were selected as the TB group, and 40 patients without tuberculosis admitted in the same period were r...

and Implications In January 1997, the USDA-FSIS mandated Escherichia coli (EC) testing in all processing plants to monitor surface contamination of beef, pork, and poultry carcasses. A novel technique that uses membrane filtration (MF) and m-ColiBlue24 (mCB) to determine the amount of EC contamination on a carcass was developed. Sponges from pork carcasses and excision samples from beef carcass...

Globally, about 1.5 billion people are infected with at least one species of soil-transmitted helminth (STH). Soil is a critical environmental reservoir of STH, yet there is no standard method for detecting STH eggs in soil. We developed a field method for enumerating STH eggs in soil and tested the method in Bangladesh and Kenya. The US Environmental Protection Agency (EPA) method for enumerat...

There is evidence to suggest that the yaws bacterium (Treponema pallidum ssp. pertenue) may exist in non-human primate populations residing in regions where yaws is endemic in humans. Especially in light of the fact that the World Health Organizaiton (WHO) recently launched its second yaws eradication campaign, there is a considerable need for reliable tools to identify treponemal infection in ...

The basic group testing problem is to identify the unknown set of " positive items " from a large population of " items " using as few " tests " as possible. A test is a subset of items. A test returns positive if there is a positive item in the subset. The semantics of " positives, " " items, " and " tests " depend on the application. In the original context [3], group testing was invented to ...

Toxoplasma gondii is a parasitic protozoan that infects nearly one-third of the world population. The present study was done to isolate and genotype T. gondii from wild boar from forests of Pahang, Malaysia. A total of 30 wild boars' blood, heads and hearts were obtained for this study and 30 (100.0%) were found to be seropositive when assayed with modified agglutination test (MAT ≥ 6). The pos...

The aim of this work was to study possibilities of using microbiological broadspectrum inhibitor test (Delvotest T) for express estimation of antibiotic residues in bovine milk. For quantitative estimation the waste-milk samples were stepwise diluted (dilution array) by antibiotic-free milk until negative test result was achieved. Another objective was to evaluate heat treatment efficiency of w...