The KiK-net is a vertical array system, and seismic motions are recorded at the ground surface and in the borehole. With the peak acceleration of 24.5 m/s2 at AKTH04 (EW) and 38.7 m/s2 at IWTH25 (UD) during the Iwate–Miyagi Nairiku earthquake in 2008, the occurrence mechanism of large accelerations is investigated by the non-stationary Fourier spectra and the double integrated displacement prof...

background: baculoviral inhibitor of apoptosis repeat-containing 5 (birc5) gene is an inhibitor of apoptosis that expresses in human embryonic tissues but it is absent in most healthy adult tissues. the copy number of birc5 has been indicated to be highly increased in tumor tissues; however, its association with the age of onset in breast cancer is not well understood. methods: forty tumor tiss...

AIMS In this study, a loop-mediated isothermal amplification (LAMP) assay has been developed and evaluated for the rapid and sensitive detection of Verticillium dahliae Kleb., the causal agent of vascular wilts in many economically important crops. METHODS AND RESULTS LAMP primers were designed based on a previously described RAPD marker, and the LAMP assay was applied for direct detection of...

To better understand the characteristics of seismic response nuclear power stations with fixed-base and pile-raft foundations, a series shaking table tests were performed. The included three cases: free-field soil, structure without superstructure foundation embedded in soil. One white noise excitation earthquake motions different intensity selected as ground motion to identify structure, raft,...

Changes in soil microbial community structure and diversity may reflect environmental impact. We examined 16S rRNA gene fingerprints of bacterial communities in six agroecosystems by PCR amplification and denaturing gradient gel electrophoresis (PCR-DGGE) separation. These soils were treated with manure for over a century or different fertilizers for over 70 years. Bacterial community structure...

In order to develop a rapid and specific detection test for bacteria in soil, we improved a method based on the polymerase chain reaction (PCR). Each step of the protocol, including direct lysis of cells, DNA purification, and PCR amplification, was optimized. To increase the efficiency of lysis, a step particularly critical for some microorganisms which resist classical techniques, we used sma...