Chromosomes isolated from mouse leukemia L1210 cells were taken up by mouse macrophages, HeLa cells, and rat embryo fibroblasts following simple exposure in vitro. The process, which resembles pinocytosis or phagocytosis, was traced by autoradiography of chromosomes prelabeled with thymidine-H(3), and by staining techniques and phase contrast microscopy. During the first six hours, the uptake o...

I T IS THE PURPOSE of this study to evaluate the uptake of tritiated thymidine ( H3TdR) by peripheral blood leukocytes as a parameter for assessing various phases of acute leukemia in remission and in relapse. Two recent reports1’2 have documented the persistence of leukemic cells in some patients with acute leukemia diagnosed as being in complete bone marrow remission after therapy. These resi...

In a series of experiments performed on intact cells or spheroplasts of E. coli and Bac. subtilis a possibility of non-thermal effects induced by continuous microwave irradiation of a low power density (at wave length range from 0.0 to 7.8 mm) was studied. Thymidine and thymine uptake, leakage of potassium and hydrogen ions as well as the uptake of the transforming DNA by the component cells of...

The characteristics of hypoxanthine transport were examined in purified brush-border membrane vesicles isolated from guineapig kidney. Hypoxanthine uptake in the vesicles was specifically stimulated by both Na+ and an inside-negative potential, resulting in a transient accumulation of intravesicular hypoxanthine. Na+-dependent hypoxanthine influx was saturable (apparent Km 4.4 + 2.1 ,uM, Vmax 1...

I T IS THE PURPOSE of this study to evaluate the uptake of tritiated thymidine ( H3TdR) by peripheral blood leukocytes as a parameter for assessing various phases of acute leukemia in remission and in relapse. Two recent reports1’2 have documented the persistence of leukemic cells in some patients with acute leukemia diagnosed as being in complete bone marrow remission after therapy. These resi...

The uptake of tritiated thymidine by lymphocytes from 100 cancer patients and 31 normal subjects was tested in the presence and absence of phytohaemagglutinin (PHA) in a medium containing autologous or homologous serum. Significant differences were found between normal subjects and cancer patients in both the time course and degree of uptake. Serum from normal individuals restored the response ...

Responses in unidirectional mixed leukocyte cultures of CBA, C3H/He, A, C57BL/6, and DBA/2 spleen cells to stimulator spleen cells derived from normal AKR mice and from AKR mice bearing a long-passage lymphoblastic leukemia (LAKR) were compared. Thymidine and uridine uptake by allogeneic cells (responder cells) cultured with either mitomycin C treated or X-irradiated LAKR cells (stimulator cell...

Responses in unidirectional mixed leukocyte cultures of CBA, C3H/He, A, C57BL/6, and DBA/2 spleen cells to stimulator spleen cells derived from normal AKR mice and from AKR mice bearing a long-passage lymphoblastic leukemia (LAKR) were compared. Thymidine and uridine uptake by allogeneic cells (responder cells) cultured with either mitomycin C treated or X-irradiated LAKR cells (stimulator cell...

The kinetic parameters of [8-(14)C]adenosine transport by a general nucleoside uptake system were studied in germinated conidia of the ad 8 strain of Neurospora crassa. The apparent K(m) for adenosine uptake by this system was found to be 6.2 muM. The apparent K(i) values for other nucleosides competing with adenosine for uptake were measured by using Dixon plots. Nucleosides which were efficie...