specific and non- specific cellular immune responses were studied in 20 amoebiasis cases and 10 controls. all the study cases and controls were indians living in varanasi, india. ten amoebic liver abscess cases were patients admitted to university hospital. ten non-invasive intestinal amoebiasis cases constituted the second study group. non-specific immune responses were studied using e-rosette...

Background: Recent studies have shown that 3′-deoxy-3′-[F] fluorothymidine ([F]FLT)) uptake depends on endogenous tumour thymidine concentration. The purpose of this study was to investigate tumour thymidine concentrations and whether they correlated with [F]FLT uptake across a broad spectrum of murine cancer models. A modified liquid chromatography-mass spectrometry (LC-MS/MS) method was used ...

BACKGROUND Recent studies have shown that 3'-deoxy-3'-[(18)F] fluorothymidine ([(18)F]FLT)) uptake depends on endogenous tumour thymidine concentration. The purpose of this study was to investigate tumour thymidine concentrations and whether they correlated with [(18)F]FLT uptake across a broad spectrum of murine cancer models. A modified liquid chromatography-mass spectrometry (LC-MS/MS) metho...

The blastic transformation in vitro of peripheral blood lymphocytes was measured by the 72-hour uptake of tritiated thymidine ((3)H-6-thymidine) in 23 patients with mucosal ulcerative colitis, three patients with acute Crohn's colitis with rectal involvement, and seven normal subjects. The 23 patients with ulcerative colitis were subdivided into three groups, graded according to severity into s...

Estimates for bacterial production in nearshore western Atlantic Ocean sediments were made using the frequency of dividing cell and thymidine uptake methods. The frequency of dividing cell (FDC) method resulted in estimates in the range of 5 to 50 g C m-' d-'. The thymidine uptake (THY) method resulted in estimates of 1 to .8 g C mP2 d-'. Metabolic measurements based on sediment oxygen uptake s...

Fluorodeoxyuridine (FdUrd) is a cytotoxic analogue of thymidine which requires activation by thymidine kinase to FdUMP. FdUMP inhibits thymidylate synthetase and, thus, the synthesis of (ITI P. 5'Aminothymidine (S'-AdThd) can antagonize the feedback inhibition ex erted by dTTP on thymidine kinase activity and thereby stimulate FdUrd phosphorylation. This provided a novel approach to assess the ...

UNLABELLED We have previously demonstrated in vitro and in vivo that tumor uptake of FDG is markedly diminished by acute hyperglycemia. This in vitro study was designed to determine if tumor uptake of PET tracers (FDG, thymidine, L-methionine and L-leucine) is affected by acute or chronic hyperglycemia. METHODS Human ovarian adenocarcinoma (HTB 77 IP3) cells were grown in media containing 100...

The influence of ribo- and deoxyribonucleosides and ribo- and deoxyribonucleotides on the uptake of radiolabeled thymidine and thymine by Haemophilus influenzae during growth was investigated. A nucleoside-degrading enzyme similar to that reported in Escherichia coli was found to break down thymidine unless other nucleosides were present to divert its action. The presence of other nucleosides p...

In the central nervous system, HIV-1 has a defined tropism for brain macrophages and microglia. Nucleoside analog drugs such as zidovudine improve the clinical and neuropsychological functions in HIV-demented patients. Multiple carrier-mediated transport systems can play an important role in the membrane permeation of nucleosides and nucleoside analog drugs in a number of cells. The purpose of ...