Cellulolytic complex are enzymes capable of hydrolyzing cellulose. Due to rapid growth in population and industrialization, most countries are required to produce more fuel. Production of bioethanol from lignocellulosic biomass is very challenging due to environmental pollution by fossil fuels. Cellulases play a significant role in biotechnological processes. The cost of production of cellulase...

A novel multi-cellulase conjugate assembled on a double-stranded DNA scaffold, a DNA-(endoglucanase)n conjugate, exhibited unique hydrolytic activity toward crystalline cellulose (Avicel) depending on the cellulase/DNA ratio on the DNA-based artificial cellulosome.

BACKGROUND Lignocellulolytic enzymes are the main enzymes to saccharify lignocellulose from renewable plant biomass in the bio-based economy. The production of these enzymes is transcriptionally regulated by multiple transcription factors. We previously engineered Penicillium oxalicum for improved cellulase production via manipulation of three genes in the cellulase expression regulatory networ...

Cellulose synthesis, but not its degradation, is generally thought to be required for plant cell growth. In this work, we cloned a dinoflagellate cellulase gene, dCel1, whose activities increased significantly in G2/M phase, in agreement with the significant drop of cellulose content reported previously. Cellulase inhibitors not only caused a delay in cell cycle progression at both the G1 and G...

Sophorose (2-O-beta-glucopyranosyl-D-glucose) induces carboxymethyl cellulase in Trichoderma reesei QM6a mycelium with 1.5 to 2 h. The induction response to sophorose concentration, although complicated by the metabolism of sophorose, shows saturation kinetics. Most of the cellulase appears after most of the sophorose has been taken up, but the presence of an inducer is required to maintain cel...

In optimization of cellulase production by different pH, substrates, nitrogen, carbon sources were detected from penicillium citrinum micro-fungal species. The maximum cellulase activity was recorded in the range of pH5 to 6.5 and wheat bran (91 U/ml) and rice bran (81 U/ml) is the best raw substrate for the production of cellulase. Estimation of extracellular protein was recorded in different ...

TiO2 photocatalyst was prepared using cellulase as a biotemplate through hydrothermal process. The as-prepared catalyst characterized based on physicochemical techniques and used to remove As(III) from contaminated water. templated (TiO2-cellulase) had large specific surface area, which reached 166.6 m2·g–1. It also strong oxidation ability and, thus, behaved well in both photocatalytic of dark...

Bovine serum albumin (BSA), polyethylene glycol (PEG) and lignosulfonate (LS) have been extensively employed as synergistic agents in lignocellulose saccharification. Nevertheless, the promoting mechanisms not fully understood there are a number of controversial opinions existed. All attention has paid to interactions between respective additive substrate. However, rarely additives enzymes (cel...

In Trichoderma reesei light is an important factor in the regulation of glycoside hydrolase gene expression. We therefore investigated the influence of different light intensities on cellulase activity and protein secretion. Differentially secreted proteins in light and darkness as identified by mass spectrometry included members of different glycoside hydrolase families, such as CBH1, Cel3A, C...