The prostaglandin H D-isomerase of rat immune accessory cells has been purified from spleen by a simple procedure, and its high specificity and activity [Urade, Fujimoto, Ujihara and Hayaishi (1987) J. Biol. Chem. 262, 3820-3825] have been confirmed in an assay coupled to prostaglandin H synthase. The enzyme also decreases the formation of 12[S]-hydroxy-5,8,10-heptadecatrienoic acid formed by t...

Glutathione S-transferase activities have been identified in the small intestine of the rat. Thrree activities obtained with p-nitrobenzyl chloride (aralkyl), 1,2-epoxy-3(p-nitrophenoxy)propane (epoxide), and ethacrynic acid (alkene) as substrates were present in significant amounts. Gel filtration indicated an elution volume for the intestinal transferase activities that was similar to those a...

The activity of eleven enzymes involved in development, ageing and in metabolism of xenobiotics in insects, was investigated under the influence of 2,4-D (2,4-Dichlorophenoxyacetic acid), in the mustard aphid, Lipaphis erysimi (Kaltenbach) (Rhynchota Aphididae), after treating the 2 instar nymphs for 13, 25 and 37 h. Studies of two hydrolases and one group transfer enzyme revealed significant e...

Evidence is presented suggesting the presence of galactosyl transferases and galactosyl acceptors on the outer surfaces of intact Balb/c 3T3 cells. In addition, the data indicate that these transferases may only be capable of transferring galactose from uridine diphosphate galactose to galactosyl acceptors on adjacent cells after intercellular contact is made (trans-glycosylation). Intact Balb/...

Significance T cells recognize their targets via T-cell receptors (TCRs), which in the case of CD8 + bind to MHC-I:antigen complexes on surface target cells. Many cancer evade immune recognition and killing by down-regulating MHC-I AgPPM. Here, we show how histone acetyl transferases p300/CBP together with NF-κB epigenetically regulate expression molecules, immunoproteasome subunits, peptide tr...

Glutathione S-transferases (GSTs) are proteins synthesized in plants and responsible for their tolerance to environmental stresses. However, little information is available on the GST gene family of sweet potato, a globally important crop. The genetic evolution GSTs potato remains unclear. present study investigated by transcriptomic comparative genomic analyses. A total 51 were identified. Gen...

Using a readily available form of the mono-phosphine ligand, Gorlos-Phos·HBF4, Pd-catalyzed borylation of aryl chlorides afforded aryl boronates in high yields. A variety of functional groups are well compatible with this palladium catalyzed borylation reaction.

A chemoselective hydrosilylation method for aldehydes is developed using a ruthenium catalyst [(Ru(p-cymene)Cl2)2] and triethylsilane; a mono hydride bridged dinuclear complex [{(η(6)-p-cymene)RuCl}2(μ-H-μ-Cl)] and a Ru(iv) mononuclear dihydride complex [(η(6)-p-cymene)Ru(H)2(SiEt3)2] are identified as potential intermediates in the reaction and the proposed catalytic cycle involves a 1,3-hydri...

Functionalization of N-heterocyclic carbenes (NHCs) has an important influence on their stability, Lewis donor, and acceptor properties. In this study, we report on the selective functionalization of a four-membered N-heterocyclic bis-silylene (2,6-Ar2C6H3NSi:)2 (1) (Ar = 2,4,6-iPr3C6H2) with mono-oxygen sources N2O and Me3NO. Treatment of 1 with N2O results in the selective formation of mono-s...

ATP(CTP):tRNA nucleotidyl transferases, tRNA maturing enzymes found in all organisms, and eubacterial poly(A) polymerases, enzymes involved in mRNA degradation, are so similar that until now their biochemical functions could not be distinguished by their amino acid sequence. BLAST searches and analysis with the program "Sequence Space" for the prediction of functional residues revealed sequence...