A cloned, permuted DNA copy of the Abelson murine leukemia virus (A-MuLV) genome was capable of eliciting the morphological transformation of NIH/3T3 fibroblasts when applied to cells in a calcium phosphate precipitate. The efficiency of the process was extremely low, yielding approximately one transformant per microgram of DNA under conditions which give 10(4) transfectants per microgram of ot...

China has been stressing the needs of promoting regional sustainable development through industrial transformation. In the northwest region of China, which is faced with both urgent socioeconomic development and fragile ecological conditions, with water scarcity being one of the major characters, the relationship between industrial transformation and water use efficiency ought to be investigate...

Maize Agrobacterium-mediated transformation efficiency has been greatly improved in recent years. Antioxidants, such as, cysteine, can significantly improve maize transformation frequency through improving the Agrobacterium infection efficiency. However, the mechanism underlying the transformation improvement after cysteine exposure has not been elucidated. In this study, we showed that the add...

The receptor tyrosine kinase Eyk, a member of the Axl/Tyro3 subfamily, activates the STAT pathway and transforms cells when constitutively activated. Here, we compared the potentials of the intracellular domains of Eyk molecules derived from c-Eyk and v-Eyk to transform rat 3Y1 fibroblasts. The v-Eyk molecule induced higher numbers of transformants in soft agar and stronger activation of Stat3;...

Different plant cultivars of the same genus and species can exhibit vastly different genetic transformation efficiencies. However, the genetic factors underlying these differences in transformation rate remain largely unknown. In barley, 'Golden Promise' is one of a few cultivars reliable for Agrobacterium-mediated transformation. By contrast, cultivar 'Haruna Nijo' is recalcitrant to genetic t...

The efficiency and robustness of reliability methods are two important factors in the first-order reliability method (FORM). The conjugate choice control (CCC) and directional chaos control method (DCC) are developed to improve the robustness and efficiency of the FORM formula using the stability transformation method. In this paper, the CCC and DCC methods are applied for the reliability analy...

In order to evaluate the effects of different pretreatments on microbial transformation of saponins in Dioscorea zingiberensis (DZW), various methods have been systematically studied on a large scale. Five pretreatments, including physical separation, catalytic solvent extraction, ultrasonic fermentation, complex enzymatic hydrolyzation and enzymatic saccharification, were performed on DZW. Com...

olonies but not from pUB110-transformed colonies (Fig. ). This is consistent with correct expression of the xynA ene contained in pUBxynA. This is the first report of the transformation of B. ubtilis using particle bombardment of submicrometer old particles. The technique offers a means for testing ransformation of recalcitrant bacteria when transformaion has not been possible through other mea...

the phase transformation phenomenon due to the crystallographic change of shape memory alloys subjected to mechanical or thermal loading is very complicated. regarding the thermo-mechanical coupling effects in shape memory alloys, in case of high  loading rates, heat generation/absorption during the forward/reverse transformation, will lead in temperature-dependent variation and consequently af...