Four cell surface-exposed, integral membrane proteins from H4-II-E-3 hepatoma monolayer cultures, derivatized by the membrane-impermeant reagent sulfosuccinimidyl 2-(biotin-amido)ethyl-1,3-dithioproprionate, were resistant to extraction with Triton X-100 at 0 degrees C. Thirty-three other similarly derivatized proteins were solubilized under these same conditions. Antisera were prepared that re...

A porous composite of cerium oxide and gama aluminum oxide pigments were prepared via sol-gel processing controlled within reverse micelles of nonionic surfactant Triton X-114 in cyclohexane. The precursor in heated at several calcinations temperature between 823 to 1123K. This process includes three steps. In the first step of preparation the ceria sol was prepared. In the second step, cyclohe...

The principal ion in the ionosphere of Triton is N+. Energetic electrons of magnetospheric origin are the primary source of ionization, with a smaller contribution due to photoionization. To explain the topside plasma scale height, we postulate that N+ ions escape from Triton. The loss rate is 3.4 x 10(7) cm-2 s-1 or 7.9 x 10(24) ions s-1. Dissociative recombination of N2+ produces neutral ...

In this work the acidity constants of Thiamine in water and water-Triton X-100 micelle media solutions, at 25 °C, have been determined spectrophotometrically. To evaluate the pH- absorbance data, a resolution based on the combination of soft-and hard-modeling is applied. The acidity constants of all related equilibria are estimated using the whole spectral fitting of the collected data to a...

Recently, we found that sphingomyelin bound and activated hepatitis C virus (HCV) 1b RNA polymerase (RdRp), thereby recruiting the HCV replication complex into lipid raft structures. Detergents are commonly used for resolving lipids and purifying proteins, including HCV RdRp. Here, we tested the effect of detergents on HCV RdRp activity in vitro and found that non-ionic (Triton X-100, NP-40, Tw...

Intravesical administration of Mycobacterium bovis bacillus Calmette-Guérin (BCG) continues to be a successful immunotherapy for superficial bladder cancer. Recently, workers in our laboratory observed expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on neutrophils in voided urine following BCG therapy. Neutrophils released a soluble and functional form of TRAIL whe...

We studied the effect in vitro of various concentrations of Triton WR-1339 on normolipidemic canine plasma and on the high density lipoproteins (HDL) isolated from this plasma by ultracentrifugation. As a preamble to this study, we established that Triton WR-1339 has a unimer molecular weight of 4,500, a micellar molecular weight of 180,000, and a critical micellar concentration (CMC) of 0.018 ...

Measurements of the surface tensions, densities and viscosities of aqueous solutions of Triton X-100 (TX-100) and rhamnolipid (RL) mixtures, at constant concentration of RL or TX-100, were carried out. The measured values of the surface tension were compared to those determined using different theoretical models and on the basis of the surface tension of aqueous solutions of individual surfacta...

A simultaneous determination of cationic and nonionic surfactants has been developed using ion-association titration. Tetrabromophenolphthalein ethyl ester (TBPE) was used as an indicator. Benzalkonium reacted with TBPE to form a blue ion-associate in the organic phase. When tetrakis(4-fluorophenyl)borate was added dropwise to the solution, the color of the organic phase turned to yellow at the...