10 While human gut microbiomes vary significantly in taxonomic composition, biological 11 pathway abundance is surprisingly invariable across hosts. We hypothesized that healthy 12 microbiomes appear functionally redundant due to factors that obscure differences in gene 13 abundance across hosts. To account for these biases, we developed a powerful test of gene 14 variability, applicable to sho...

conclusions this study provides initial data on the prevalence and distribution of the flaa, cadf, ciab, cdtb, cgtb, docc and wlan genes in c. jejuni. background campylobacter jejuni (c. jejuni) is a major cause of human diarrheal disease. objectives this study was conducted to determine the prevalence of different pathogenic genes in isolates recovered from human stool samples in rosario, arge...

Genetic polymorphisms in diagnostic antigens are important factors responsible for variable performance of rapid diagnostic tests. Additionally, the failure of antigen expression due to gene deletion may also contribute to variable performance. We report Indian Plasmodium falciparum field isolates lacking both Pfhrp2 and Pfhrp3 genes leading to false negative results of rapid diagnostic tests. ...

Immunoglobulin genes can be efficiently expressed following transfection into myeloma cells. Using protoplast fusion, transfection frequencies greater than 10(-3) can be achieved. Compatible plasmids containing two different selectible markers are used to simultaneously deliver heavy and light chain genes to the same cell. To produce molecules with differing specificities the rearranged and exp...

Large tracts of the human genome, known as gene deserts, are devoid of protein-coding genes. Dichotomy in their level of conservation with chicken separates these regions into two distinct categories, stable and variable. The separation is not caused by differences in rates of neutral evolution but instead appears to be related to different biological functions of stable and variable gene deser...

background and objectives: enteropathogenic escherichia coli (epec) divided into two groups typical and atypical (aspect). the main virulence genes are located in a pathogenicity island called lee ( locus of enterocyte effacement ). lee frequently inserted in trna genes of selc , pheu and phev in the bacterial chromosome. tepec and aepec strains have some differences in their pathogenicity. the...

although more than 98% of the human genome is transcribed, most of these transcripts are not translated into proteins. rather, they are considered as non-coding rnas. micrornas (mirnas) are very short non-coding rnas approximately 22 nucleotides in length which regulate many key processes of cells such as growth, proliferation, differentiation, cell cycle, apoptosis (programmed cell death) and ...

The use of polymorphic markers in breeding programmes could make selection more accurate and efficient. A total of 324 individuals from six Iranian cattle populations (Sarabi, Golpayegani, Sistani, Taleshi, Mazandarani, Dashtiyari), F1 Golpayegani × Brown Swiss and Iranian buffalo populations were genotypedfor the Pit-1 HinfI and leptin Sau3AI polymorphisms by the polymerase chain reactio...